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Invitrogen
PDPK1 Recombinant Rabbit Monoclonal Antibody (SD204-9)
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Product Details
MA5-32354
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SD204-9
Immunogen
Synthetic peptide within Human PDPK1 aa 1-50.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; mPDK1; OTTHUMP00000174525; OTTHUMP00000205076; PDPK1; PkB kinase; PkB kinase like gene 1; PkB-like 1; predicted protein of HQ0461; Protein kinase B kinase; Putative 3-phosphoinositide-dependent protein kinase 2; unnamed protein product
Gene Aliases: PDK1; PDPK1; PDPK2; PDPK2P; PRO0461
UniProt ID: (Human) O15530, (Mouse) Q9Z2A0, (Rat) O55173
Entrez Gene ID: (Human) 5170, (Mouse) 18607, (Rat) 81745
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
3-phosphoinositide-dependent protein kinase activity
protein binding
ATP binding
phospholipase activator activity
kinase activity
transferase activity
phospholipase binding
protein serine kinase activity
insulin receptor binding
transferase activity, transferring phosphorus-containing groups
protein kinase binding
non-receptor serine/threonine protein kinase
type B pancreatic cell development
protein phosphorylation
hyperosmotic response
epidermal growth factor receptor signaling pathway
insulin receptor signaling pathway
regulation of endothelial cell migration
negative regulation of cardiac muscle cell apoptotic process
cell migration
calcium-mediated signaling
actin cytoskeleton organization
negative regulation of transforming growth factor beta receptor signaling pathway
T cell costimulation
cellular response to insulin stimulus
negative regulation of toll-like receptor signaling pathway
intracellular signal transduction
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mast cell degranulation
protein kinase B signaling
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
cellular response to epidermal growth factor stimulus
extrinsic apoptotic signaling pathway
vascular endothelial cell response to laminar fluid shear stress
intracellular signaling cassette
positive regulation of protein localization to plasma membrane
positive regulation of sprouting angiogenesis
positive regulation of vascular endothelial cell proliferation
negative regulation of endothelial cell apoptotic process
transcription, DNA-templated
regulation of transcription, DNA-templated
negative regulation of protein kinase activity
signal transduction
positive regulation of phospholipase activity
phosphorylation
peptidyl-threonine phosphorylation
activation of protein kinase B activity
focal adhesion assembly
positive regulation of establishment of protein localization to plasma membrane
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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