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Invitrogen
Phospho-c-Raf (Ser621) Recombinant Rabbit Monoclonal Antibody (JJ085-05)
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FIGURE: 1 / 6
Phospho-c-Raf (Ser621) Antibody (MA5-32438) in IHC (P)
Immunohistochemistry analysis of Phospho-c-Raf (Ser621) with paraffin-embedded human kidney. The tissues were blocked in 1% BSA (30 min, room temp), washed with ddH2O and PBS, incubated with recombinant monoclonal Phospho-c-Raf (Ser621) (Product # MA5-32438) at a dilution of 1:400 (30 min, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-32438
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JJ085-05
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser621 of Human Raf1 aa 591-640.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-RAF; C-Raf proto-oncogene, serine/threonine kinase; cRAF; Craf1 transforming; EC 2.7.11.1; kinase Raf1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; proto-oncogene c-RAF; raf protein; raf proto-oncogene serine/threonine protein kinase; unnamed protein product; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; v-raf-leukemia viral oncogene 1; vraf1 murine le
Gene Aliases: 6430402F14Rik; c-Raf; CMD1NN; CRAF; Craf1; D830050J10Rik; NS5; Raf-1; v-Raf
UniProt ID: (Human) P04049, (Mouse) Q99N57, (Rat) P11345
Entrez Gene ID: (Human) 5894, (Mouse) 110157, (Rat) 24703
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
adenylate cyclase activator activity
enzyme binding
small GTPase binding
identical protein binding
protein serine kinase activity
adenylate cyclase binding
mitogen-activated protein kinase kinase binding
macromolecular complex binding
MAPK cascade
response to hypoxia
cellular glucose homeostasis
positive regulation of protein phosphorylation
protein phosphorylation
signal transduction
neuromuscular junction development
negative regulation of cell proliferation
insulin receptor signaling pathway
Schwann cell development
cell differentiation
thyroid gland development
negative regulation of protein complex assembly
positive regulation of peptidyl-serine phosphorylation
somatic stem cell population maintenance
intracellular signal transduction
insulin secretion involved in cellular response to glucose stimulus
response to muscle stretch
ERBB signaling pathway
ERBB2-ERBB3 signaling pathway
myelination
negative regulation of apoptotic process
positive regulation of MAPK cascade
intermediate filament cytoskeleton organization
positive regulation of transcription from RNA polymerase II promoter
insulin-like growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
thymus development
face development
death-inducing signaling complex assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
heart development
apoptotic process
regulation of Rho protein signal transduction
wound healing
regulation of apoptotic process
regulation of cell differentiation
interferon-gamma-mediated signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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