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ARID1A Recombinant Rabbit Monoclonal Antibody (JJ09-01)
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FIGURE: 1 / 12
ARID1A Antibody (MA5-32465) in ICC/IF
Immunofluorescence of ARID1A was performed using HAP1 WT (lane 1) and ARID1A KO (lane 2) cells that were labeled with CellTracker Green CMFDA (Product # C2925) or CellTracker™ Deep Red (Product # C34565), respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goa... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-32465
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JJ09-01
Immunogen
Recombinant protein within Human ARID1A aa 1,184-1,334.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARID domain-containing protein 1A; ARID family protein; AT rich interactive domain 1A (SWI-like); AT rich interactive domain 1A (Swi1 like); brain protein 120; BRG1-associated factor 250a; chromatin remodeling factor p250; h BM029; osa homolog 1; OSA1 nuclear protein; SNF/SWI complex largest subunit; SWI-like protein; SWI/SNF complex protein p270; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; unnamed protein product
Gene Aliases: 1110030E03Rik; B120; BAF250; BAF250a; BM029; C1orf4; CSS2; ELD; hELD; hOSA1; MRD14; OSA1; P270; SMARCF1
UniProt ID: (Human) O14497, (Mouse) A2BH40
Entrez Gene ID: (Human) 8289, (Mouse) 93760, (Rat) 297867
negative regulation of transcription from RNA polymerase II promoter
formation of primary germ layer
neural tube closure
cardiac chamber development
optic cup formation involved in camera-type eye development
chromatin organization
nucleosome disassembly
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
gastrulation
embryo implantation
stem cell population maintenance
regulation of mitotic metaphase/anaphase transition
forebrain development
positive regulation of T cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
positive regulation of transcription, DNA-templated
cardiac muscle cell differentiation
placenta blood vessel development
regulation of G0 to G1 transition
positive regulation of stem cell population maintenance
regulation of G1/S transition of mitotic cell cycle
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
positive regulation of gene expression, epigenetic
biological_process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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