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ADAM17 Recombinant Rabbit Monoclonal Antibody (JM10-35)

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ADAM17 Recombinant Rabbit Monoclonal Antibody (JM10-35)

Product Details

MA5-32572

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
- -

Immunohistochemistry (Paraffin) (IHC (P))

1:500
-

Immunohistochemistry (Frozen) (IHC (F))

1:100
-

Immunocytochemistry (ICC/IF)

1:100-1:200
-

Flow Cytometry (Flow)

1:500-1:1,000
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JM10-35

Immunogen

Synthetic peptide within Human ADAM17 aa 757-790.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809849

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

ADAM17 (disintegrin and metalloproteinase domain-containing protein 17) cleaves the membrane bound precusor of TNF-alpha to its mature soluble form. ADAM 17 is responsbile for the proteolytical release of soluble JAM3 from endothelial cells surface and the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1, and the amyloid precursor protein. It acts as an activator of the Notch pathway by mediating cleavage of Notch and generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Mutations in the gene can result in inflammatory skin and bowel disease, neonatal 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: a disintegrin and metallopeptidase domain 17; a disintegrin and metalloprotease domain 17; a disintegrin and metalloproteinase; a disintegrin and metalloproteinase 17; a disintegrin and metalloproteinase domain 17; a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme); ADAM; ADAM 17; ADAM metallopeptidase domain 18; ADAMs; cartilage snake venom-like protease; CD156b; CD156b antigen; Disintegrin and metalloproteinase domain-containing protein 17; metalloendopeptidases; Snake venom-like protease; TNF-alpha convertase; TNF-alpha convertase enzyme; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme; tumor overexpression; unnamed protein product

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Gene Aliases: ADAM17; ADAM18; CD156B; CSVP; NISBD; NISBD1; TACE

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UniProt ID: (Human) P78536, (Mouse) Q9Z0F8, (Rat) Q9Z1K9

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Entrez Gene ID: (Human) 6868, (Mouse) 11491, (Rat) 57027

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Function(s)
endopeptidase activity metalloendopeptidase activity Notch binding interleukin-6 receptor binding integrin binding protein binding peptidase activity metallopeptidase activity hydrolase activity SH3 domain binding cytokine binding PDZ domain binding tumor necrosis factor binding metal ion binding metallodipeptidase activity metalloendopeptidase activity involved in amyloid precursor protein catabolic process zinc ion binding protease
Process(es)
response to hypoxia neutrophil mediated immunity germinal center formation production of molecular mediator involved in inflammatory response positive regulation of leukocyte chemotaxis proteolysis membrane protein ectodomain proteolysis cell adhesion epidermal growth factor receptor signaling pathway Notch signaling pathway Notch receptor processing positive regulation of cell proliferation response to xenobiotic stimulus positive regulation of signal transduction positive regulation of T cell chemotaxis negative regulation of neuron projection development protein processing signal release B cell differentiation positive regulation of cell growth positive regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway response to lipopolysaccharide positive regulation of chemokine production positive regulation of tumor necrosis factor production regulation of mast cell apoptotic process T cell differentiation in thymus cell adhesion mediated by integrin wound healing, spreading of epidermal cells amyloid precursor protein catabolic process protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of epidermal growth factor receptor signaling pathway spleen development regulation of axon regeneration cell motility defense response to Gram-positive bacterium cellular response to high density lipoprotein particle stimulus commissural neuron axon guidance negative regulation of cold-induced thermogenesis cytokine precursor processing signaling receptor ligand precursor processing positive regulation of G1/S transition of mitotic cell cycle positive regulation of tumor necrosis factor-mediated signaling pathway positive regulation of vascular endothelial cell proliferation regulation of neuron migration positive regulation of protein phosphorylation positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway response to drug negative regulation of apoptotic process positive regulation of epidermal growth factor-activated receptor activity PMA-inducible membrane protein ectodomain proteolysis positive regulation of cellular component movement response to high density lipoprotein particle integrin-mediated signaling pathway negative regulation of interleukin-8 production
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