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Invitrogen
CD59 (Protectin) Recombinant Rabbit Monoclonal Antibody (JM10-71)
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FIGURE: 1 / 4
CD59 (Protectin) Antibody (MA5-32588) in WB
Western Blot was performed using Anti-CD59 (Protectin) Recombinant Rabbit Monoclonal Antibody (JM10-71) (Product # MA5-32588) and an 18 kDa band corresponding to CD59 glycoprotein was observed across all the cell lines and tissue lysates tested.. Membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), SK-BR-3 (Lane 2), T-47D (Lane 3), HeLa (Lane 4), A549 (Lane 5), U-937 (Lane 6), Caco-2 (Lane 7) and Mouse Lung (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then... View More
Product Details
MA5-32588
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM10-71
Immunogen
Synthetic peptide within Human CD59 (aa 85-121).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). CD59 is the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 also associates with C5b-8 complex and counteracts appropriate formation of cytolytic pore within the plasma membrane. Further, CD59 is a low-affinity ligand of human CD2, causes T cell costimulation, and is involved in lymphocyte signal transduction. CD59 is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. Mutations in this gene cause CD59 deficiency, a disease involving hemolytic anemia, thrombosis, and cerebral infarction. Multiple alternatively spliced transcript variants of CD59 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1F5 antigen; 20 kDa homologous restriction factor; antigen CD59 precursor (CD59); CD59; CD59 antigen; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 blood group antigen; CD59 glycoprotein; CD59 molecule, complement regulatory protein; CD59A glycoprotein; CD59B glycoprotein; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MAC-inhibitory protein; MAC-IP; MACIF; MEM43 antigen; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MIRL; Protectin; surface anitgen recognized by monoclonal antibody 16.3A5; T cell-activating protein; unnamed protein product
Gene Aliases: 16.3A5; 1F5; AA987121; CD59; Cd59a; Cd59b; EJ16; EJ30; EL32; G344; HRF-20; HRF20; MAC-IP; MACIF; MEM43; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20; protectin
UniProt ID: (Human) P13987, (Mouse) O55186, (Mouse) P58019
Entrez Gene ID: (Human) 966, (Mouse) 12509, (Mouse) 333883
negative regulation of activation of membrane attack complex
cell surface receptor signaling pathway
negative regulation of angiogenesis
negative regulation of vascular endothelial growth factor receptor signaling pathway
negative regulation of complement activation
negative regulation of fibroblast growth factor production
positive regulation of T cell proliferation
negative regulation of apoptotic process
negative regulation of cytolysis
complement activation
blood coagulation
regulation of complement activation
regulation of complement-dependent cytotoxicity
negative regulation of complement-dependent cytotoxicity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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