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Invitrogen
B-Raf Monoclonal Antibody (F1-F4-E10)
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FIGURE: 1 / 16
B-Raf Antibody (MA5-32846) in ICC/IF
Immunofluorescence analysis of BRAF was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 1 hour, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with B-Raf Monoclonal Antibody (F1-F4-E10) (Product # MA5-32846, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:2,... View More
Product Details
MA5-32846
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
F1-F4-E10
Immunogen
Recombinant protein within Human B-Raf aa 49-239.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 50% Glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in B-Raf have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for B-Raf.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; FLJ95109; MGC126806; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; serine/threonine protein kinase; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1
Gene Aliases: 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; B-RAF1; BRAF; BRAF-1; Braf-2; BRAF1; Braf2; C230098H17; C87398; D6Ertd631e; NS7; RAFB1
UniProt ID: (Human) P15056, (Mouse) P28028
Entrez Gene ID: (Human) 673, (Rat) 114486, (Mouse) 109880
nucleotide binding
catalytic activity
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase activity
MAP kinase kinase kinase activity
calcium ion binding
protein binding
ATP binding
zinc ion binding
kinase activity
transferase activity
small GTPase binding
identical protein binding
metal ion binding
scaffold protein binding
protein serine kinase activity
mitogen-activated protein kinase kinase binding
protein heterodimerization activity
receptor signaling protein activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
MAPK cascade
myeloid progenitor cell differentiation
protein phosphorylation
signal transduction
epidermal growth factor receptor signaling pathway
visual learning
organ morphogenesis
positive regulation of gene expression
negative regulation of fibroblast migration
positive regulation of glucose transport
synaptic vesicle exocytosis
cell differentiation
thyroid gland development
T cell differentiation in thymus
positive regulation of peptidyl-serine phosphorylation
substrate adhesion-dependent cell spreading
somatic stem cell population maintenance
regulation of cell proliferation
negative regulation of apoptotic process
stress fiber assembly
CD4-positive, alpha-beta T cell differentiation
positive T cell selection
CD4-positive or CD8-positive, alpha-beta T cell lineage commitment
negative regulation of neuron apoptotic process
regulation of T cell differentiation
alpha-beta T cell differentiation
thymus development
regulation of axon regeneration
positive regulation of axon regeneration
positive regulation of axonogenesis
T cell receptor signaling pathway
positive regulation of stress fiber assembly
long-term synaptic potentiation
head morphogenesis
face development
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to calcium ion
cellular response to xenobiotic stimulus
endothelial cell apoptotic process
establishment of protein localization to membrane
postsynaptic modulation of chemical synaptic transmission
positive regulation of substrate adhesion-dependent cell spreading
negative regulation of synaptic vesicle exocytosis
negative regulation of endothelial cell apoptotic process
activation of MAPKK activity
cellular response to drug
response to peptide hormone
protein heterooligomerization
response to cAMP
glucose transport
phosphorylation
intracellular signal transduction
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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