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Invitrogen
NME1 Monoclonal Antibody (12A1)
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FIGURE: 1 / 4
NME1 Antibody (MA5-34934) in IHC (P)
Immunohistochemistry analysis of NM23 in paraffin-embedded human thyroid gland tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with NM23 monoclonal antibody (Product # MA5-34934) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counterstai... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-34934
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
12A1
Immunogen
Synthetic peptide within Human NM23 aa 78-126.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: MCF-7, human breast cancer tissue, human liver cancer tissue, human thyroid gland tissue.
Target Information
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; farnesyl diphosphate kinase NME1; Granzyme A activated DNase (GAAD); granzyme A-activated DNase; GZMA activated DNase; histidine protein kinase NME1; included; metastasis inhibition factor nm23; NDP kinase A; NM23H1; NM23H1B; NME1; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; non-specific serine/threonine protein kinase NME1; nucleoside diphoshate kinase 1; putative 3'-5'-DNA exonuclease NDK1; putative granzyme A-activated DNA endonuclease; tumor metastatic process-associated protein; unnamed protein product
Gene Aliases: AWD; GAAD; NB; NBS; NDK1; NDKA; NDPK A; NDPK-A; NDPKA; NM23; NM23-H1
UniProt ID: (Human) P15531
Entrez Gene ID: (Human) 4830
magnesium ion binding
DNA binding
RNA binding
endodeoxyribonuclease activity
deoxyribonuclease activity
nucleoside diphosphate kinase activity
protein histidine kinase activity
protein serine/threonine kinase activity
protein binding
3'-5'-exodeoxyribonuclease activity
kinase activity
phosphotransferase activity, phosphate group as acceptor
GDP binding
identical protein binding
ribosomal small subunit binding
ADP binding
farnesyl diphosphate kinase activity
protein serine kinase activity
coenzyme A binding
succinyl-CoA binding
acetyl-CoA binding
GTP biosynthetic process
UTP biosynthetic process
CTP biosynthetic process
base-excision repair
DNA catabolic process
apoptotic DNA fragmentation
isoprenoid metabolic process
nucleoside phosphate metabolic process
negative regulation of cell proliferation
granzyme-mediated apoptotic signaling pathway
nucleoside diphosphate metabolic process
nucleoside triphosphate biosynthetic process
protein hexamerization
regulation of fatty acid biosynthetic process
regulation of apoptotic process
ITP biosynthetic process
acetyl-CoA catabolic process
positive regulation of epithelial cell proliferation
purine-containing compound metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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