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Invitrogen
LC3A Recombinant Rabbit Monoclonal Antibody (7F5R1)
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FIGURE: 1 / 2
LC3A Antibody (MA5-35164) in WB
Western blot analysis of LC3A in SH-SY5Y cells. Samples were incubated with LC3A Monoclonal antibody (Product # MA5-35164) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-35164
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7F5R1
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.4 mg/mL
Amount
40 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE
Target Information
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: autophagy-related ubiquitin-like modifier LC3 A; LC3-like protein 1; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; Microtubule-asso; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A; MLP3A; RP11-346K17.1
Gene Aliases: ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
UniProt ID: (Human) Q9H492
Entrez Gene ID: (Human) 84557
autophagosome assembly
mitophagy
mitophagy
autophagy
cellular response to nitrogen starvation
JNK cascade
cellular response to starvation
response to iron(II) ion
response to lead ion
macroautophagy
response to nutrient levels
cellular response to amino acid starvation
p38MAPK cascade
SMAD protein signal transduction
cellular response to hydrogen peroxide
cellular response to copper ion
cellular response to oxygen-glucose deprivation
autophagosome maturation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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