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Invitrogen
Caspase 2 Precursor Recombinant Rabbit Monoclonal Antibody (2Z10F1)
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FIGURE: 1 / 1
Caspase 2 Precursor Antibody (MA5-35599) in WB
Western blot analysis of Caspase 2 Precursor in extracts of various cell lines. Samples were incubated with Caspase 2 Precursor Monoclonal antibody (Product # MA5-35599) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Jurkat cells were treated by Staurosporine (1 µM) for 3 hours. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-35599
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2Z10F1
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 334-452 of human Caspase-2 (P29594)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.05 mg/mL
Amount
205 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: GKNHTQSPGC EESDAGKEEL MKMRLPTRSD MICGYACLKG NAAMRNTKRG SWYIEALTQV FSERACDMHV ADMLVKVNAL IKEREGYAPG TEFHRCKEMS EYCSTLCQQL YLFPGYPPT
Target Information
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CASP2; caspase 2 apoptosis-related cysteine peptidase; ICH-1L; ICH-1L/1S; neural precursor cell expressed developmentally down-regulated protein 2; protease ICH-1; protein phosphatase 1, regulatory subunit 57; unnamed protein product
Gene Aliases: CASP-2; ICH1; MRT80; NEDD-2; NEDD2; PPP1R57
UniProt ID: (Human) P42575
Entrez Gene ID: (Human) 835
luteolysis
release of cytochrome c from mitochondria
neural retina development
proteolysis
apoptotic process
cellular response to DNA damage stimulus
intrinsic apoptotic signaling pathway in response to DNA damage
protein processing
DNA damage response, signal transduction by p53 class mediator
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of neuron apoptotic process
protein maturation
negative regulation of necroptotic process
cellular response to mechanical stimulus
apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
execution phase of apoptosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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