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Invitrogen

PROX1 Recombinant Rabbit Monoclonal Antibody (9I9A7)

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PROX1 Recombinant Rabbit Monoclonal Antibody (9I9A7)

Product Details

MA5-35749

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800
-

Immunocytochemistry (ICC/IF)

1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

9I9A7

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PROX1 (Q92786)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849649

Product Specific Information

Immunogen sequence: MPDHDSTALL SRQTKRRRVD IGVKRTVGTA SAFFAKARAT FFSAMNPQGS EQDVEYSVVQ HADGEKSNVL RKLLKRANSY EDAMMPFPGA TIISQLLKNN

Target Information

The expression pattern of the Prox1 homeo box gene suggests that it has a role in a variety of embryonic tissues, including lens. Analysis of mRNA reveals that Prox mRNA is present in many different human tissues and that lens demonstrated the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. The Prox1 gene is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been document to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Homeobox prospero-like protein PROX1; Prospero homeobox protein 1; prospero-related homeobox 1; PROX-1; unnamed protein product

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Gene Aliases: A230003G05Rik; PROX1

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UniProt ID: (Human) Q92786, (Mouse) P48437

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Entrez Gene ID: (Human) 5629, (Rat) 305066, (Mouse) 19130

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding protein binding ligand-dependent nuclear receptor binding sequence-specific DNA binding DBD domain binding LBD domain binding sequence-specific double-stranded DNA binding core promoter sequence-specific DNA binding molecular_function transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity transcription regulatory region DNA binding transcription factor activity, sequence-specific DNA binding homeodomain transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter kidney development liver development positive regulation of endothelial cell proliferation lymph vessel development lymphangiogenesis lens development in camera-type eye lens morphogenesis in camera-type eye hepatocyte cell migration regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter neuroblast proliferation negative regulation of neuroblast proliferation central nervous system development brain development circadian rhythm cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation regulation of gene expression positive regulation of endothelial cell migration epithelial cell migration dorsal spinal cord development dentate gyrus development cerebellar granule cell differentiation neural tube development skeletal muscle thin filament assembly lung development olfactory placode formation pancreas development response to nutrient levels tube development regulation of circadian rhythm otic placode formation negative regulation of viral genome replication cell fate commitment positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter optic placode formation involved in camera-type eye formation rhythmic process anatomical structure formation involved in morphogenesis neuron fate determination inner ear development venous blood vessel morphogenesis ventricular cardiac myofibril assembly atrial cardiac muscle tissue morphogenesis ventricular cardiac muscle tissue morphogenesis retina morphogenesis in camera-type eye embryonic retina morphogenesis in camera-type eye endocardium formation positive regulation of sarcomere organization ventricular septum morphogenesis aorta smooth muscle tissue morphogenesis positive regulation of heart growth lymphatic endothelial cell differentiation blood vessel endothelial cell differentiation lymphatic endothelial cell fate commitment branching involved in pancreas morphogenesis lens fiber cell morphogenesis hepatocyte differentiation negative regulation of bile acid biosynthetic process hepatocyte proliferation acinar cell differentiation neuronal stem cell population maintenance positive regulation of vascular endothelial growth factor signaling pathway positive regulation of cell cycle checkpoint positive regulation of neural precursor cell proliferation positive regulation of forebrain neuron differentiation cell fate determination negative regulation of sequence-specific DNA binding transcription factor activity endothelial cell differentiation positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of transcription, DNA-templated regulation of transcription involved in lymphatic endothelial cell fate commitment transcription, DNA-templated multicellular organism development
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