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Phospho-Tau (Ser396) Recombinant Rabbit Monoclonal Antibody (5M1O6)
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FIGURE: 1 / 4
Phospho-Tau (Ser396) Antibody (MA5-35871) in IHC (P)
Immunohistochemistry analysis of Phospho-Tau (Ser396) in paraffin-embedded mouse brain. Samples were incubated with Phospho-Tau (Ser396) Monoclonal antibody (Product # MA5-35871) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-35871
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5M1O6
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: YKSPVV
Target Information
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ31424; FTDP17; map tau; MGC138549; microtubules; neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; paired helical filament-tau; PHFtau; tau microtubule binding protein; Tau-4; Tau5; unnamed protein product
Gene Aliases: Mtapt; PHF-tau; Tau
UniProt ID: (Mouse) P10637
Entrez Gene ID: (Mouse) 17762
DNA binding
enzyme inhibitor activity
protein binding
microtubule binding
tubulin binding
SH3 domain binding
enzyme binding
protein kinase binding
heat shock protein binding
apolipoprotein binding
identical protein binding
macromolecular complex binding
chaperone binding
protein phosphatase 2A binding
Hsp90 protein binding
lipoprotein particle binding
microtubule lateral binding
microtubule stabilizing activity
microtubule cytoskeleton organization
neuron migration
cellular response to DNA damage stimulus
axonogenesis
synapse assembly
response to nutrient
learning or memory
memory
adult walking behavior
axo-dendritic transport
intrinsic apoptotic signaling pathway in response to oxidative stress
response to lead ion
regulation of autophagy
negative regulation of gene expression
negative regulation of mitochondrial membrane potential
positive regulation of mitochondrial membrane potential
positive regulation of neuron projection development
regulation of microtubule polymerization or depolymerization
positive regulation of microtubule polymerization
neuron projection development
negative regulation of intracellular transport
positive regulation of superoxide anion generation
positive regulation of axon extension
microtubule polymerization
mitochondrion transport along microtubule
intracellular distribution of mitochondria
axon extension
synapse organization
regulation of calcium-mediated signaling
mRNA transport
protein polymerization
protein homooligomerization
response to hyperoxia
regulation of microtubule-based movement
regulation of microtubule cytoskeleton organization
negative regulation of mitochondrial fission
fibril organization
regulation of cellular response to heat
positive regulation of long term synaptic depression
positive regulation of protein localization to synapse
negative regulation of establishment of protein localization to mitochondrion
positive regulation of cellular protein localization
negative regulation of tubulin deacetylation
positive regulation of mesenchymal stem cell migration
amyloid fibril formation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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