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Invitrogen

Phospho-CDK1/CDK2/CDK3 (Thr14) Recombinant Rabbit Monoclonal Antibody (4I9L3)

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Phospho-CDK1/CDK2/CDK3 (Thr14) Recombinant Rabbit Monoclonal Antibody (4I9L3)

Product Details

MA5-35888

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4I9L3

Immunogen

A synthetic phosphorylated peptide around T14 of human CDK1/2/3CDK1 (P06493)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Amount

80 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849786

Product Specific Information

Immunogen sequence: EGTYG

Target Information

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1- mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1- dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the 2 centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis (PubMed:26549230).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cdc2-related protein kinase; CDK2; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; cell division control protein 2 homolog; cell division cycle 2 homolog A; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; cell division protein kinase 1; cell division protein kinase 2; cell division protein kinase 3; cyclin dependent kinase 2-alpha; p33 protein kinase; p34 protein kinase; unnamed protein product

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Gene Aliases: 2310050O10Rik; A630093N05Rik; CDC2; CDC28A; Cdc2a; Cdk3-ps; CDKN2; p33(CDK2); p34; P34CDC2

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UniProt ID: (Human) P06493, (Human) P24941, (Human) Q00526, (Mouse) P11440, (Rat) P39951, (Mouse) P97377

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Entrez Gene ID: (Human) 983, (Human) 1017, (Human) 1018, (Mouse) 12534, (Rat) 54237, (Rat) 362817, (Mouse) 12566, (Mouse) 69681

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Function(s)
chromatin binding protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity cyclin binding Hsp70 protein binding histone kinase activity cyclin-dependent protein kinase activity protein serine kinase activity magnesium ion binding protein domain specific binding macromolecular complex binding
Process(es)
G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle cellular response to DNA damage stimulus signal transduction cell proliferation G0 to G1 transition negative regulation of Notch signaling pathway regulation of cell cycle microtubule cytoskeleton organization DNA replication DNA repair chromatin remodeling regulation of transcription from RNA polymerase II promoter mitotic nuclear envelope disassembly mitotic G2 DNA damage checkpoint centrosome cycle pronuclear fusion response to toxic substance positive regulation of gene expression positive regulation of G2/M transition of mitotic cell cycle regulation of Schwann cell differentiation response to amine response to activity cell migration protein deubiquitination peptidyl-threonine phosphorylation chromosome condensation epithelial cell differentiation negative regulation of protein ubiquitination protein localization to kinetochore positive regulation of protein import into nucleus response to hydrogen peroxide regulation of circadian rhythm negative regulation of apoptotic process response to ethanol positive regulation of DNA replication positive regulation of smoothened signaling pathway regulation of embryonic development response to cadmium ion response to copper ion response to axon injury ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation positive regulation of mitotic sister chromatid segregation protein-containing complex assembly cellular response to hydrogen peroxide ERK1 and ERK2 cascade Golgi disassembly regulation of heterochromatin organization positive regulation of protein localization to nucleus regulation of mitotic attachment of spindle microtubules to kinetochore microtubule cytoskeleton organization involved in mitosis positive regulation of mitochondrial ATP synthesis coupled electron transport positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process protein phosphorylation nuclear envelope organization negative regulation of gene expression peptidyl-serine phosphorylation mitotic cell cycle phase transition thymidine biosynthetic process microtubule polymerization cilium disassembly DNA strand resection involved in replication fork processing DNA synthesis involved in mitotic DNA replication protein localization to site of double-strand break mitotic DNA-dependent DNA replication negative regulation of transcription from RNA polymerase II promoter lens development in camera-type eye potassium ion transport centriole replication Ras protein signal transduction positive regulation of cell proliferation response to xenobiotic stimulus positive regulation of heterochromatin assembly positive regulation of DNA-dependent DNA replication initiation response to estradiol cellular response to insulin stimulus negative regulation of protein catabolic process telomere maintenance in response to DNA damage post-translational protein modification positive regulation of transcription, DNA-templated response to cAMP response to electrical stimulus liver regeneration negative regulation of protein localization to chromatin positive regulation of meiotic cell cycle process involved in oocyte maturation meiotic spindle organization meiotic cell cycle process involved in oocyte maturation regulation of mitotic cell cycle mitotic G1 DNA damage checkpoint centrosome duplication meiotic cell cycle cellular response to nitric oxide cellular senescence regulation of anaphase-promoting complex-dependent catabolic process telomere maintenance protection from non-homologous end joining at telomere
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