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          • Primary Antibodies ›
          • RhoA Antibodies

          Invitrogen

          RhoA Monoclonal Antibody (2148CT124.4.9)

          View all (29) RhoA antibodies

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          Cite RhoA Monoclonal Antibody (2148CT124.4.9)

          Additional Information:

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          • Antibody Testing Data (1)
          RhoA Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          RhoA Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          RhoA Antibody (MA5-37789) in WB

          Western Blot analysis of RhoA using the RhoA Monoclonal Antibody (Product # MA5-37789) Lane 1: PC-12 whole cell lysate, Lane 2: NIH/3T3 whole cell lysate, Lane 3: L929 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          RhoA Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          MA5-37789

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:160,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2148CT124.4.9

          Immunogen

          recombinant protein from human RhoA

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2897713

          Target Information

          RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Aplysia ras-related homolog 12; aplysia ras-related homolog A; epididymis secretory sperm binding protein; MGC72339; oncogene RHO H12; plysia ras-related homolog A2; ras homolog gene family, member A; Rho; RhoA; small GTP binding protein RhoA; unnamed protein product

          View more View less

          Gene Aliases: ARH12; ARHA; Arha1; Arha2; EDFAOB; RHO12; RHOH12

          View more View less

          UniProt ID: (Human) P61586, (Rat) P61589, (Mouse) Q9QUI0

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          Entrez Gene ID: (Human) 387, (Rat) 117273, (Mouse) 11848

          View more View less

          Function(s)
          GTPase activity obsolete small monomeric GTPase activity protein binding GTP binding myosin binding protein kinase binding GDP binding protein domain specific binding Rho GDP-dissociation inhibitor binding
          Process(es)
          mitotic cytokinesis alpha-beta T cell lineage commitment aortic valve formation small GTPase mediated signal transduction Rho protein signal transduction negative regulation of cell-substrate adhesion cell migration substantia nigra development actin cytoskeleton organization regulation of cell migration positive regulation of cell migration cytoplasmic microtubule organization positive regulation of cytokinesis regulation of actin cytoskeleton organization regulation of osteoblast proliferation cell junction assembly substrate adhesion-dependent cell spreading Roundabout signaling pathway cleavage furrow formation apolipoprotein A-I-mediated signaling pathway odontogenesis positive regulation of I-kappaB kinase/NF-kappaB signaling stress fiber assembly apical junction assembly beta selection endothelial cell migration ossification involved in bone maturation wound healing, spreading of cells establishment of epithelial cell apical/basal polarity positive regulation of neuron differentiation negative regulation of cell size positive regulation of alpha-beta T cell differentiation negative chemotaxis positive regulation of stress fiber assembly regulation of focal adhesion assembly Wnt signaling pathway, planar cell polarity pathway positive regulation of lipase activity bone trabecula morphogenesis regulation of microtubule cytoskeleton organization cellular response to lipopolysaccharide cellular response to cytokine stimulus semaphorin-plexin signaling pathway positive regulation of protein serine/threonine kinase activity negative regulation of cell migration involved in sprouting angiogenesis mitotic spindle assembly negative regulation of oxidative phosphorylation endothelial tube lumen extension regulation of modification of postsynaptic structure positive regulation of NIK/NF-kappaB signaling skeletal muscle satellite cell migration negative regulation of reactive oxygen species biosynthetic process mitotic cleavage furrow formation positive regulation of leukocyte adhesion to vascular endothelial cell regulation of modification of postsynaptic actin cytoskeleton cellular response to chemokine positive regulation of T cell migration meiotic spindle organization cell morphogenesis response to hypoxia kidney development angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure regulation of systemic arterial blood pressure by endothelin regulation of transcription from RNA polymerase II promoter cytoskeleton organization microtubule depolymerization cell adhesion cell-matrix adhesion skeletal muscle tissue development regulation of actin polymerization or depolymerization response to xenobiotic stimulus response to mechanical stimulus response to glucose regulation of neuron projection development negative regulation of neuron projection development cerebral cortex cell migration forebrain radial glial cell differentiation cell differentiation positive regulation of cell growth androgen receptor signaling pathway positive regulation of actin filament polymerization establishment or maintenance of actin cytoskeleton polarity stress-activated protein kinase signaling cascade negative regulation of intracellular steroid hormone receptor signaling pathway embryonic cleavage polar body extrusion after meiotic divisions negative regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process response to ethanol negative regulation of neuron differentiation positive regulation of translation positive regulation of cell adhesion positive regulation of vasoconstriction positive regulation of smooth muscle contraction GTP metabolic process neuron projection morphogenesis regulation of dendrite development response to glucocorticoid spindle localization regulation of calcium ion transport negative regulation of synapse assembly cellular response to progesterone stimulus positive regulation of podosome assembly positive regulation of vascular smooth muscle contraction negative regulation of vascular smooth muscle cell proliferation negative regulation of vascular associated smooth muscle cell migration regulation of neural precursor cell proliferation integrin-mediated signaling pathway
          It has to be done as per old AB suggested Products section.

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