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Invitrogen
PHF8 Recombinant Rabbit Monoclonal Antibody (2H9D4)
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FIGURE: 1 / 2
PHF8 Antibody (MA5-37995) in WB
Western blot analysis of PHF8 in extracts of various cell lines. Samples were incubated with PHF8 Monoclonal antibody (Product # MA5-37995) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-37995
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2H9D4
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human PHF8 (Q9UPP1)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HeLa, C2C12, Mouse thymus
Immunogen sequence: TVSNSPASQR TPGKRPIKRP AYWRTESEEE EENASLDEQD SLGACFKDAE YIYPSLESDD DDPALKSRPK KKKNSDDAPW SPKARVTPTL PKQDRPVREG T
Target Information
PHD finger protein 8 is a jumonji domain containing protein. Like other members of the jumonji family, PHF8 may therefore play a role in histone demethylation. Mutations in PHF8 lead to Siderius type X-linked mental retardation (MRXSSD), a mild to borderline type of mental retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-dimethyl-L-lysine(36) demethylase PHF8; [histone H3]-dimethyl-L-lysine(9) demethylase PHF8; Histone lysine demethylase PHF8; jumonji C domain-containing histone demethylase 1F; Lysine demethylase 7B; PHD finger protein 8; unnamed protein product; zinc finger protein 422
Gene Aliases: 9830141C09Rik; JHDM1F; KDM7B; KIAA1111; mKIAA1111; MRXSSD; PHF8; RGD1561065; ZNF422
UniProt ID: (Human) Q9UPP1, (Mouse) Q80TJ7
Entrez Gene ID: (Human) 23133, (Mouse) 320595, (Rat) 317425
chromatin binding
transcription cofactor activity
iron ion binding
protein binding
zinc ion binding
oxidoreductase activity
histone demethylase activity
histone demethylase activity (H3-K9 specific)
histone demethylase activity (H4-K20 specific)
metal ion binding
dioxygenase activity
histone demethylase activity (H3-K36 specific)
histone demethylase activity (H3-K27 specific)
histone H3K4me3 reader activity
histone H3-di/monomethyl-lysine-36 demethylase activity
histone H3K9me/H3K9me2 demethylase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
methylated histone binding
molecular_function
G1/S transition of mitotic cell cycle
histone H3-K9 demethylation
histone H4-K20 demethylation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase I promoter
oxidation-reduction process
negative regulation of chromatin silencing at rDNA
histone H3-K36 demethylation
histone H3-K27 demethylation
biological_process
chromatin organization
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
brain development
positive regulation of transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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