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          • Primary Antibodies ›
          • p53 Antibodies

          Invitrogen

          Acetyl-p53 (Lys370) Recombinant Rabbit Monoclonal Antibody (9X9X3)

          View all (266) p53 antibodies

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          Cite Acetyl-p53 (Lys370) Recombinant Rabbit Monoclonal Antibody (9X9X3)

          Additional Information:
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          • Antibody Testing Data (2)
          Acetyl-p53 (Lys370) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Acetyl-p53 (Lys370) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Acetyl-p53 (Lys370) Antibody (MA5-38031) in WB

          Western blot analysis of Acetyl-p53 (Lys370) in extracts of various cell lines. Samples were incubated with Acetyl-p53 (Lys370) Monoclonal antibody (Product # MA5-38031) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa cells and NIH/3T3 cells were treated by TSA (1 µM) at 37°C for 18 hours. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Acetyl-p53 (Lys370) Antibody in Western Blot (WB)
          Acetyl-p53 (Lys370) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Acetyl-p53 (Lys370) Recombinant Rabbit Monoclonal Antibody (9X9X3)

          Product Details

          MA5-38031

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          9X9X3

          Immunogen

          A synthetic acetylated peptide around K370 of human p53 (P04637)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.7 mg/mL

          Amount

          170 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2897949

          Product Specific Information

          Positive Samples: HeLa, NIH/3T3, C6

          Immunogen sequence: GGSRAHSSHL KSKKGQSTSR H

          Target Information

          The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: antigen NY-CO-13; Cys 51 Stop; FLJ92943; HGNC11998; ME-delta123p53; MLLP53; mutant; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p53; p53 antigen; p53 cellular tumor antigen; phosphoprotein p53; transcription factor; transcription factor; truncated protein; transformation related protein 53; transformation-related protein 53; tumor protein 53; tumor protein p53 (Li-Fraumeni syndrome); tumor suppressor; tumor suppressor p53; tumor supressor p53; Tumour Protein p53; unnamed protein product

          View more View less

          Gene Aliases: bbl; BCC7; bfy; bhy; BMFS5; LFS1; p44; P53; Tp53; TRP53

          View more View less

          UniProt ID: (Human) P04637, (Rat) P10361, (Mouse) P02340

          View more View less

          Entrez Gene ID: (Human) 7157, (Rat) 24842, (Mouse) 22059

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          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding TFIID-class transcription factor binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding mRNA 3'-UTR binding copper ion binding protein binding zinc ion binding enzyme binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone deacetylase regulator activity annealing helicase activity identical protein binding histone deacetylase binding protein heterodimerization activity chaperone binding protein phosphatase 2A binding RNA polymerase II sequence-specific DNA binding transcription factor binding 14-3-3 protein binding MDM2/MDM4 family protein binding disordered domain specific binding general transcription initiation factor binding molecular function activator activity molecular condensate scaffold activity promoter-specific chromatin binding transcription cofactor binding sequence-specific DNA binding ion channel binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter hematopoietic progenitor cell differentiation positive regulation of leukocyte migration nucleotide-excision repair regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter autophagy apoptotic process cellular response to DNA damage stimulus response to oxidative stress ER overload response Ras protein signal transduction spermatogenesis circadian rhythm protein localization negative regulation of cell proliferation determination of adult lifespan mRNA transcription response to xenobiotic stimulus response to UV response to metal ion response to X-ray response to UV-B response to gamma radiation positive regulation of gene expression viral process negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator response to caffeine mitotic G1 DNA damage checkpoint negative regulation of telomere maintenance via telomerase regulation of prostaglandin secretion response to retinoic acid tumor necrosis factor-mediated signaling pathway response to vitamin B3 response to cytokine cellular response to heat cellular response to reactive oxygen species cellular response to UV cellular response to glucose starvation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process entrainment of circadian clock by photoperiod response to amino acid response to ethanol positive regulation of cell cycle positive regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly positive regulation of transcription from RNA polymerase II promoter response to antibiotic negative regulation of fibroblast proliferation circadian behavior bone marrow development negative regulation of smooth muscle cell proliferation negative regulation of DNA metabolic process protein tetramerization regulation of intracellular pH regulation of cell cycle response to hyperoxia hematopoietic stem cell differentiation interferon-gamma-mediated signaling pathway positive regulation of programmed necrotic cell death protein-containing complex assembly intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress positive regulation of thymocyte apoptotic process cellular response to triglyceride cellular response to hypoxia cellular response to xenobiotic stimulus cellular response to ionizing radiation cellular response to gamma radiation signal transduction by p53 class mediator intrinsic apoptotic signaling pathway by p53 class mediator cellular response to actinomycin D positive regulation of release of cytochrome c from mitochondria regulation of peptide hormone secretion cellular senescence replicative senescence oxidative stress-induced premature senescence apoptotic signaling pathway oligodendrocyte apoptotic process positive regulation of execution phase of apoptosis response to benzene regulation of cell cycle G2/M phase transition positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of G1 to G0 transition cellular response to polyamine macromolecule positive regulation of outer hair cell apoptotic process negative regulation of pentose-phosphate shunt negative regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process positive regulation of cellular senescence negative regulation of progesterone secretion regulation of intrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway in utero embryonic development somitogenesis release of cytochrome c from mitochondria T cell proliferation involved in immune response B cell lineage commitment T cell lineage commitment response to ischemia DNA repair double-strand break repair protein import into nucleus transforming growth factor beta receptor signaling pathway regulation of mitotic cell cycle gastrulation negative regulation of neuroblast proliferation central nervous system development heart development negative regulation of DNA replication rRNA transcription response to salt stress embryo development ending in birth or egg hatching gene expression negative regulation of gene expression positive regulation of cardiac muscle cell apoptotic process cerebellum development negative regulation of transforming growth factor beta receptor signaling pathway T cell differentiation in thymus cellular response to stress regulation of tissue remodeling multicellular organism growth positive regulation of mitochondrial membrane permeability regulation of cell proliferation mitochondrial DNA repair regulation of DNA damage response, signal transduction by p53 class mediator regulation of neuron apoptotic process positive regulation of neuron apoptotic process negative regulation of proteolysis negative regulation of mitotic cell cycle fibroblast proliferation cell development embryonic organ development protein stabilization chromosome organization neuron apoptotic process cardiac septum morphogenesis regulation of thymocyte apoptotic process necroptotic process cellular response to UV-C negative regulation of macromitophagy regulation of mitochondrial membrane permeability involved in apoptotic process regulation of intrinsic apoptotic signaling pathway by p53 class mediator negative regulation of production of miRNAs involved in gene silencing by miRNA negative regulation of glucose catabolic process to lactate via pyruvate intrinsic apoptotic signaling pathway in response to hypoxia regulation of fibroblast apoptotic process negative regulation of reactive oxygen species metabolic process regulation of cellular senescence response to ionizing radiation negative regulation of helicase activity intrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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