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Invitrogen
EGR2 Recombinant Rabbit Monoclonal Antibody (1F6V7)
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FIGURE: 1 / 2
EGR2 Antibody (MA5-38053) in WB
Western blot analysis of EGR2 in extracts of various cell lines. Samples were incubated with EGR2 Monoclonal antibody (Product # MA5-38053) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-38053
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1F6V7
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EGR2 (NP_0003902)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: PC-3, SH-SY5Y, Mouse brain, Rat brain
Immunogen sequence: MMTAKAVDKI PVTLSGFVHQ LSDNIYPVED LAATSVTIFP NAELGGPFDQ MNGVAGDGMI NIDMTGEKRS LDLPYPSSFA PVSAPRNQTF TYMGKFSIDP
Target Information
The EGR2 gene provides instructions for making a protein called early growth response 2, which is part of the early growth response family of proteins. These proteins bind to specific areas of DNA and help control the activity of particular genes. On the basis of this action, the proteins are referred to as transcription factors. The early growth response 2 protein activates several genes that are involved in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells. Myelin promotes the efficient transmission of nerve impulses. If myelin is lost (demyelination) or its structure is disrupted, the transmission of nerve impulses is impaired. Mutations in the EGR2 gene can cause two forms of Charcot-Marie-Tooth disease, type 1D or type 4E (sometimes called congenital hypomyelinating neuropathy) or a severe form of type 1D (sometimes called Dejerine-Sottas syndrome) that begins during infancy or early childhood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AT591; DKFZp686J1957; E3 SUMO-protein ligase EGR2; E3 SUMO-protein transferase ERG2; early growth response 2 protein; Early growth response protein 2; EGR-2; FLJ1454; FLJ14547; Krox-20 homolog; KROX-20, Drosophila, homolog (early growth response-2); OTTHUMP00000062133; unnamed protein product; Zinc finger protein Krox-20
Gene Aliases: AT591; CMT1D; CMT4E; Egr-2; EGR2; Krox-20; KROX20; NGF1-B; Zfp-25; Zfp-6
UniProt ID: (Human) P11161, (Mouse) P08152, (Rat) P51774
Entrez Gene ID: (Human) 1959, (Mouse) 13654, (Rat) 114090
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
transferase activity
ubiquitin protein ligase binding
sequence-specific DNA binding
metal ion binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
SUMO ligase activity
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
nucleic acid binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
transcription regulatory region DNA binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
HMG box domain binding
C2H2 zinc finger transcription factor
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein export from nucleus
brain development
peripheral nervous system development
rhythmic behavior
motor neuron axon guidance
gene expression
Schwann cell differentiation
positive regulation of Schwann cell differentiation
protein sumoylation
rhombomere 3 development
facial nerve structural organization
rhombomere 3 structural organization
rhombomere 3 formation
rhombomere 5 structural organization
rhombomere 5 formation
regulation of ossification
positive regulation of myelination
brain segmentation
aorta development
skeletal muscle cell differentiation
myelination
fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
transcription from RNA polymerase II promoter
response to insulin
negative regulation of apoptotic process
regulation of neuronal synaptic plasticity
cellular response to organic substance
cellular response to cAMP
cellular response to gonadotropin stimulus
learning or memory
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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