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Invitrogen
MDH1 Recombinant Rabbit Monoclonal Antibody (8U9X1)
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FIGURE: 1 / 6
MDH1 Antibody (MA5-38200) in ICC/IF
Immunofluorescence of MDH1 was performed using HAP1 WT (lane 1) and MDH1 KO (lane 2) cells that were labeled with CellTracker Green CMFDA (Product # C2925) or CellTracker™ Deep Red (Product # C34565), respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat se... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-38200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8U9X1
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.16 mg/mL
Amount
16 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HeLa, Jurkat, U-251MG, Mouse liver, Mouse brain, Rat kidney, Rat heart
Immunogen sequence: IKARKLSSAM SAAKAICDHV RDIWFGTPEG EFVSMGVISD GNSYGVPDDL LYSFPVVIKN KTWKFVEGLP INDFSREKMD LTAKELTEEK ESAFEFLSSA
Target Information
MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aromatic alpha-keto acid reductase; cytosolic malate dehydrogenase; diiodophenylpyruvate reductase; epididymis secretory protein Li 32; malate dehydrogenase 1, NAD (soluble); malate dehydrogenase, soluble; Malate dehydrogenase-like enzyme; MDH1; soluble malate dehydrogenase; unnamed protein product
Gene Aliases: B230377B03Rik; DEE88; EIEE88; HEL-S-32; KAR; MDH-s; MDHA; Mdhl; MDL1; MGC:1375; Mor-2; MOR2
UniProt ID: (Human) P40925, (Mouse) P14152, (Rat) O88989
Entrez Gene ID: (Human) 4190, (Mouse) 17449, (Rat) 24551
catalytic activity
malic enzyme activity
protein binding
oxidoreductase activity
malate dehydrogenase activity
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
L-malate dehydrogenase activity
2-hydroxyglutarate dehydrogenase activity
hydroxyphenylpyruvate reductase activity
NAD binding
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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