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Invitrogen
Bub1 Recombinant Rabbit Monoclonal Antibody (9C11)
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FIGURE: 1 / 1
Bub1 Antibody (MA5-38345) in WB
Western Blot analysis of Bub1 using Bub1 Monoclonal Antibody (Product # MA5-38345) at 1:1,000. Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 123, 116, 120 kDa. Observed band size: 130 kDa
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-38345
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9C11
Immunogen
A synthesized peptide derived from human Bub1 (aa 1-182).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.25 mg/mL
Amount
125 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody has been tested in direct-ELISA
Target Information
The BUB1 gene encodes a serine/threonine-protein kinase that is integral to the spindle assembly checkpoint, a crucial mechanism ensuring proper chromosome alignment and segregation during mitosis. BUB1 is involved in detecting and correcting errors in kinetochore-microtubule attachments, which is vital for maintaining genomic stability. It acts by delaying the onset of anaphase until all chromosomes are correctly attached to the spindle apparatus. BUB1 is recruited to kinetochores in early mitosis, where it phosphorylates several substrates to activate other checkpoint proteins, such as BUBR1 and MAD2, thereby preventing premature progression through mitosis. Mutations or aberrations in the expression levels of BUB1 are often associated with aneuploidy and chromosomal instability, contributing to cancer progression. BUB1 is thus considered a potential target for cancer therapeutics, aiming to exploit its role in the mitotic checkpoint to selectively eliminate tumor cells through checkpoint bypass or mitotic arrest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BUB1 budding uninhibited by benzimidazoles 1 homolog; budding uninhibited by benzimidazoles 1 homolog; huBUB1; HuBub1; putative serine/threonine-protein kinase; mitotic spindle checkpoint kinase; protein kinase; putative serine/threonine-protein kinase; unnamed protein product
Gene Aliases: BUB1A; BUB1L; hBUB1; MCPH30
UniProt ID: (Human) O43683
Entrez Gene ID: (Human) 699
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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