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Invitrogen
African Swine Fever Virus Structural Protein p54 Monoclonal Antibody (GT1075)
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FIGURE: 1 / 6
African Swine Fever Virus Structural Protein p54 Antibody (MA5-42320) in ICC/IF
African Swine Fever Virus Structural Protein p54 Monoclonal Antibody (GT1075) detects ASFV p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASFV p54 stained by African Swine Fever Virus Structural Protein p54 Monoclonal Antibody (GT1075) (Product # MA5-42320) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-42320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT1075
Immunogen
Recombinant protein encompassing a sequence within the center region of ASFV p54 (ASFV (strain: BA71V)). The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target Information
The African Swine Fever Virus (ASFV) structural protein p54, encoded by the E183L gene, is a crucial component of the virus's architecture, playing a significant role in its morphogenesis. This 25-kDa polypeptide is essential for the recruitment of envelope precursors to assembly sites, contributing to the formation of viral particles. It is highly antigenic and has been localized within the replication factories of infected cells, predominantly associated with membranes and immature virus particles. The protein p54 has been shown to interact with target cells, aiding in the virus's attachment and entry, alongside other viral proteins like p30. Attempts to inactivate the p54 gene have demonstrated its necessity for ASFV replication, underscoring its potential as a target for therapeutic interventions. Its structural and functional properties make p54 a focal point of study in the development of ASFV treatments and prevention strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: African swine fever virus p54
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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