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Invitrogen
ICAD Recombinant Rabbit Monoclonal Antibody (JE49-23)
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FIGURE: 1 / 6
ICAD Antibody (MA5-42485) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded human pancreas intestine tissue using ICAD Recombinant Monoclonal Antibody (Product # MA5-42485). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the ICAD anti... View More
Product Details
MA5-42485
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE49-23
Immunogen
Recombinant protein within human ICAD aa 37-137.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Jurkat cell lysate, human stomach tissue lysate, human thyroid tissue, human breast tissue, human small intestine tissue, human pancreas intestine tissue, HCT116.
Subcellular Location: Cytoplasm.
Target Information
ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DFF; DFF-45; DFF45; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD; nuclease inhibitor; RP23-121D17.3; unnamed protein product
Gene Aliases: DFF-45; DFF1; DFF45; DFFA; H13; ICAD
UniProt ID: (Human) O00273
Entrez Gene ID: (Human) 1676
apoptotic DNA fragmentation
protein folding
apoptotic process
negative regulation of deoxyribonuclease activity
regulation of apoptotic process
positive regulation of apoptotic process
thymocyte apoptotic process
negative regulation of execution phase of apoptosis
negative regulation of apoptotic DNA fragmentation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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