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Invitrogen
UBE2B Recombinant Rabbit Monoclonal Antibody (2Z3R2)
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FIGURE: 1 / 5
UBE2B Antibody (MA5-42600) in IHC (P)
Immunohistochemistry analysis of UBE2B in paraffin-embedded human esophageal cancer. Samples were incubated with UBE2B Monoclonal antibody (Product # MA5-42600) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-42600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2Z3R2
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.33 mg/mL
Amount
33 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HeLa, 293T, NIH/3T3, K-562, Mouse testis, Mouse heart, Rat testis. The target is usually found in the following locations: Cell membrane, Nucleus.
Immunogen sequence: FKLVIEFSEE YPNKPPTVRF LSKMFHPNVY ADGSICLDIL QNRWSPTYDV SSILTSIQSL LDEPNPNSPA NSQAAQLYQE NKREYEKRVS AIVEQSWNDS
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2 protein; E2 ubiquitin-conjugating enzyme B; E2(14k); HHR6B (Human homologue of yeast RAD 6); putative; RAD6 homolog B; UBE2B; ubiquitin carrier protein B; ubiquitin conjugating enzyme; ubiquitin conjugating enzyme E2B; ubiquitin-conjugating enzyme E2(14k); ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); ubiquitin-conjugating enzyme E2B (RAD6 homolog, S. cerevisiae); ubiquitin-conjugating enzyme E2B, RAD6 homolog; ubiquitin-conjugating enzyme E2B, RAD6 homology; ubiquitin-protein ligase B; unnamed protein product
Gene Aliases: E2-14k; E2-17kDa; E214K; HHR6B; HR6B; mHR6B; RAD6B; UBC2
UniProt ID: (Human) P63146, (Mouse) P63147, (Rat) P63149
Entrez Gene ID: (Human) 7320, (Mouse) 22210, (Rat) 81816
protein polyubiquitination
in utero embryonic development
DNA repair
postreplication repair
chromatin organization
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
spermatogenesis
sperm axoneme assembly
response to xenobiotic stimulus
response to UV
positive regulation of reciprocal meiotic recombination
protein ubiquitination
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
meiotic telomere clustering
protein stabilization
chiasma assembly
protein autoubiquitination
synaptonemal complex organization
protein K63-linked ubiquitination
protein K48-linked ubiquitination
protein K11-linked ubiquitination
positive regulation of canonical Wnt signaling pathway
negative regulation of post-translational protein modification
response to hypoxia
cellular response to insulin stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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