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Invitrogen
UBE4A Recombinant Rabbit Monoclonal Antibody (2A5N2)
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FIGURE: 1 / 2
UBE4A Antibody (MA5-42621) in WB
Western blot analysis of UBE4A in extracts of various cell lines. Samples were incubated with UBE4A Monoclonal antibody (Product # MA5-42621) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-42621
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2A5N2
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE4A (Q14139).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.2 mg/mL
Amount
120 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HUVEC, Mouse brain, Mouse kidney, Rat liver, Rat brain. The target is usually found in the following locations: Cytoplasm.
Immunogen sequence: MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS DNSVSESLDE FDYSVAEISR SFRSQQEICE QLNINHMIQR IFLITLDNSD
Target Information
Ubiquitination requires a ubiquitin-activating enzyme, a ubiquitin-conjugating enzyme, and usually a substrate-specific ubiquitin-protein ligase. Multiubiquitination needs a fourth factor, to bind to the ubiquitin moieties of preformed conjugates and catalyze ubiquitin chain assembly in conjunction with the first three proteins. Human protein KIAA0126, with a predicted 1,073 amino acid residues, is a member of the novel protein family defined by ubiquitination conjugation factor E4. In yeast, E4 activity is linked to cell survival under stress conditions, indicating that eucaryotes use E4-dependent proteolysis pathways for multiple cellular functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: KIAA0126; liver regeneration-related protein LRRG073; RING-type E3 ubiquitin transferase E4 A; ubiquitination factor E4A (UFD2 homolog, yeast); ubiquitination factor E4A, UFD2 homolog; unnamed protein product
Gene Aliases: 4732444G18Rik; 9930123J21Rik; Ab2-232; E4; NEDHMS; UBOX2; UFD2; UFD2b
UniProt ID: (Human) Q14139, (Rat) Q6P7A2, (Mouse) E9Q735
Entrez Gene ID: (Human) 9354, (Rat) 315608, (Mouse) 140630
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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