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Invitrogen
IDO Recombinant Rabbit Monoclonal Antibody (6W2F7)
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FIGURE: 1 / 2
IDO Antibody (MA5-42653) in IHC (P)
Immunohistochemistry analysis of IDO in paraffin-embedded mouse spinal cord. Samples were incubated with IDO Monoclonal antibody (Product # MA5-42653) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-42653
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6W2F7
Immunogen
Synthetic peptide.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.8 mg/mL
Amount
80 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: Mouse ovary.
Immunogen sequence: IESGQLRERV EKLNMLSIDH LTDHKSQRLA RLVLGCITMA YVWGKGHGDV RKVLPRNIAV PYCQLSKKLE LPPILVYADC VLANWKKKDP NKPLTYENMD V
Target Information
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 1.13.11.52; IDO-1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; indoleamine 2,3-dioxygenase (IDO) (EC 1.13.11.17); Indoleamine-2; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; unnamed protein product
Gene Aliases: IDO; IDO-1; INDO
UniProt ID: (Human) P14902, (Mouse) P28776
Entrez Gene ID: (Human) 3620, (Mouse) 15930
tryptophan 2,3-dioxygenase activity
electron carrier activity
heme binding
indoleamine 2,3-dioxygenase activity
metal ion binding
protein binding
amino acid binding
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
oxygen binding
tryptophan catabolic process
female pregnancy
quinolinate biosynthetic process
'de novo' NAD biosynthetic process from tryptophan
regulation of activated T cell proliferation
positive regulation of T cell apoptotic process
positive regulation of T cell tolerance induction
positive regulation of chronic inflammatory response
positive regulation of type 2 immune response
defense response
inflammatory response
response to lipopolysaccharide
negative regulation of interleukin-10 production
positive regulation of interleukin-12 production
multicellular organismal response to stress
kynurenic acid biosynthetic process
swimming behavior
negative regulation of T cell proliferation
positive regulation of apoptotic process
negative regulation of activated T cell proliferation
negative regulation of T cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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