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ASK Recombinant Rabbit Monoclonal Antibody (0S8I5)
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FIGURE: 1 / 1
ASK Antibody (MA5-42736) in WB
Western blot analysis of ASK in extracts of various cell lines. Samples were incubated with ASK Monoclonal antibody (Product # MA5-42736) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-42736
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0S8I5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DBF4 (Q9UBU7).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.35 mg/mL
Amount
35 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: PC-3, Jurkat.
Immunogen sequence: MNSGAMRIHS KGHFQGGIQV KNEKNRPSLK SLKTDNRPEK SKCKPLWGKV FYLDLPSVTI SEKLQKDIKD LGGRVEEFLS KDISYLISNK KEAKFAQTLG
Target Information
Activator of S phase kinase (ASK, DBF4) is a novel regulatory subunit of the human Cdc7 kinase complex that plays a pivotal role in G1/S cell cycle transition in mammalian cells. The increase in Cdc7 kinase activity at the G1/S boundary is at least partly accounted for by the elevated expression of ASK in late G1. ASK interacts with replication origins in vivo, suggesting that Cdc7 may trigger S phase by directly activating the replication initiation complexes assembled at the origins. Human ASK protein levels are regulated during the cell cycle with a pattern that matches that of human Cdc7 protein kinase activity. Human Cdc7-ASK kinase is directly involved in regulating the initiation of DNA replication by targeting MCM2 protein in mammalian cells. Nuclear localization and chromatin binding of endogenous huCdc7 and GFP-ASK is presumably imported into nuclei through its two nuclear localization signals at telophase, it may require additional signals for chromatin binding, the level of which increases at late G1 ohase. A functional link may exist between menin, a tumor suppressor protein mutated in multiple endocrine neoplasia type I (MEN1), and ASK in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activator of S phase kinase; chiffon homolog A; DBF4 homolog; DBF4 zinc finger A; DBF4-type zinc finger-containing protein 1; HsCdc7 protein kinase regulatory subunit; regulatory subunit of DDK; unnamed protein product; zinc finger, DBF-type containing 1
Gene Aliases: ASK; CHIF; DBF4A; ZDBF1
UniProt ID: (Human) Q9UBU7
Entrez Gene ID: (Human) 10926
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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