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Invitrogen
CD86 (B7-2) Monoclonal Antibody (10)
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FIGURE: 1 / 1
CD86 (B7-2) Antibody (MA5-43049) in Flow
Flow cytometry of CD86 (B7-2) in LPS-stimulated SD rat splenocytes. Samples were incubated in CD86 (B7-2) monoclonal antibody (Product # MA5-43049) followed by FITC. Assay results were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-43049
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
10
Immunogen
Recombinant Rat CD86/B7-2 Protein. (1-250).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Specificity: Rat CD86/B7-2
Target Information
CD86, along with CD80, is a member of the B7 family of costimulatory molecules and plays a crucial role in T cell activation and immune response regulation. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and its expression is upregulated on B cells through various stimuli, including the BCR complex, CD40, and certain cytokine receptors. As a type I membrane protein and member of the immunoglobulin superfamily, CD86 serves as a ligand for the T cell surface proteins CD28 and CTLA-4 (CD152). The interaction between CD86 and CD28 provides a costimulatory signal essential for T cell activation during antigen presentation, while binding with CTLA-4 negatively regulates T cell activation, diminishing the immune response. This interaction is critical for T-B cell crosstalk, T cell costimulation, autoantibody production, and Th2-mediated Ig production. The kinetics of CD86 upregulation upon stimulation suggest its significant contribution during the primary phase of an immune response. CD86 and CD80 have distinct roles in T helper cell differentiation, and insufficient co-stimulation involving these molecules can induce tolerance. Alternative splicing of CD86 results in two transcript variants encoding different isoforms, with additional variants described but not fully sequenced. Dysfunction in CD86 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B lymphocyte activation antigen B72; CD28 antigen ligand 2; cd86 antigen; Early T-cell co-stimulatory molecule 1; membrane glycoprotein; MGC34413
Gene Aliases: B7-2
Entrez Gene ID: (Rat) 56822
response to yeast
toll-like receptor signaling pathway
T cell proliferation involved in immune response
positive regulation of immunoglobulin production
negative regulation of T cell anergy
immune response
signal transduction
cell surface receptor signaling pathway
positive regulation of cell proliferation
response to xenobiotic stimulus
positive regulation of signal transduction
T cell costimulation
response to lipopolysaccharide
toll-like receptor 3 signaling pathway
response to interferon-gamma
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
T cell activation
B cell activation
negative regulation of T cell proliferation
myeloid dendritic cell differentiation
lymphocyte activation
positive regulation of immune response
positive regulation of T cell receptor signaling pathway
regulation of T cell activation
defense response to virus
cellular response to lipopolysaccharide
cellular response to metal ion
cellular response to cytokine stimulus
positive regulation of NIK/NF-kappaB signaling
regulation of leukocyte cell-cell adhesion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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