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Invitrogen

CD45 Recombinant Rabbit Monoclonal Antibody (BL-178-12C7)

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CD45 Recombinant Rabbit Monoclonal Antibody (BL-178-12C7)

Product Details

MA5-44219

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Flow Cytometry (Flow)

1 µL/1x10^6 cells
-

Immunoprecipitation (IP)

20 µL/ mg lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

BL-178-12C7

Immunogen

Synthetic peptide representing a region between residue 1250 to 1306 (C-terminus) of human CD45 Molecule.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

50 µg/mL

Amount

5 µg

Purification

purified

Storage buffer

BBS, pH 8.2, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2926349

Product Specific Information

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD45 antigen; glycoprotein 180; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, c polypeptide; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220; unnamed protein product

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Gene Aliases: B220; CD45; CD45R; GP180; IMD105; L-CA; LCA; LY5; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)
protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity receptor binding protein binding heparin binding protein kinase binding protein tyrosine kinase inhibitor activity ankyrin binding spectrin binding heparan sulfate proteoglycan binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter natural killer cell differentiation negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation positive regulation of immunoglobulin production negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration signal transduction cell surface receptor signaling pathway response to gamma radiation regulation of gene expression dephosphorylation B cell differentiation T cell differentiation positive regulation of B cell proliferation regulation of interleukin-8 production negative regulation of interleukin-2 production positive regulation of interleukin-2 production positive regulation of tumor necrosis factor production B cell proliferation positive regulation of T cell proliferation T cell activation positive regulation of MAPK cascade cell cycle phase transition plasma membrane raft distribution positive regulation of protein kinase activity negative regulation of JAK-STAT cascade bone marrow development stem cell development regulation of phagocytosis positive regulation of phagocytosis positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway regulation of T cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of Fc receptor mediated stimulatory signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade negative regulation of interleukin-4-mediated signaling pathway negative regulation of microglial cell activation response to aldosterone DN2 thymocyte differentiation positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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