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Invitrogen
Phospho-CHK1 (Ser345) Recombinant Rabbit Monoclonal Antibody (PS01-17)
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FIGURE: 1 / 7
Phospho-CHK1 (Ser345) Antibody (MA5-45974) in ICC/IF
Immunofluorescence analysis of CHK1 (pS345) was performed using 70% confluent log phase HeLa cells treated with 5 μM Etoposide for 16 hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Phospho-CHK1 (Ser345) Recombinant Rabbit Monoclonal Antibody (PS01-... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45974
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PS01-17
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser345 of human Chk1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.081 mg/mL
Amount
108.1 µg
Purification
Protein A
Storage buffer
PBS with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Predicted band size: 54 kDa
Positive Control: HeLa treated with Etoposide cell lysate, HeLa treated with Hydroxyurea cell lysate, HeLa treated with 4mM Hydroxyurea for 20 hours.
Subcellular Location: Nucleus, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Sequence Similarities: 100% Mouse/Rat.
Tissue Specificity: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.
Target Information
CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; Synoviolin; unnamed protein product
Gene Aliases: CHK1; OZEMA21
UniProt ID: (Human) O14757
Entrez Gene ID: (Human) 1111
DNA damage checkpoint
DNA replication
DNA repair
chromatin remodeling
protein phosphorylation
cellular response to DNA damage stimulus
mitotic G2 DNA damage checkpoint
regulation of double-strand break repair via homologous recombination
peptidyl-threonine phosphorylation
signal transduction in response to DNA damage
mitotic G2/M transition checkpoint
positive regulation of cell cycle
negative regulation of gene expression, epigenetic
negative regulation of mitotic nuclear division
regulation of mitotic centrosome separation
negative regulation of G0 to G1 transition
cellular response to mechanical stimulus
replicative senescence
regulation of signal transduction by p53 class mediator
negative regulation of cell cycle phase transition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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