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Invitrogen
CYLD Recombinant Rabbit Monoclonal Antibody (HL1261)
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FIGURE: 1 / 1
CYLD Antibody (MA5-46978) in WB
Western blot analysis of CYLD in Various whole cell extracts (30 µg) separated by 7.5% SDS-PAGE. Membrane was blotted with CYLD Monoclonal antibody (Product # MA5-46978) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-46978
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL1261
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human CYLD. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
Target Information
CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cyld; cylindromatosis (turban tumor syndrome); deubiquitinating enzyme CYLD; FLJ20180; FLJ31664; FLJ78684; probable ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; ubiquitin-specific-processing protease CYLD; unnamed protein product
Gene Aliases: BRSS; CDMT; CYLD1; CYLDI; EAC; FTDALS8; MFT; MFT1; SBS; TEM; USPL2
UniProt ID: (Human) Q9NQC7
Entrez Gene ID: (Human) 1540
regulation of mitotic cell cycle
regulation of tumor necrosis factor-mediated signaling pathway
protein deubiquitination
negative regulation of type I interferon production
nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway
negative regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
negative regulation of JNK cascade
regulation of inflammatory response
negative regulation of inflammatory response
regulation of necroptotic process
necroptotic process
regulation of microtubule cytoskeleton organization
protein K63-linked deubiquitination
negative regulation of canonical Wnt signaling pathway
negative regulation of NIK/NF-kappaB signaling
regulation of cilium assembly
negative regulation of p38MAPK cascade
protein linear deubiquitination
negative regulation of interleukin-18-mediated signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
regulation of intrinsic apoptotic signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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