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Invitrogen
Nestin Monoclonal Antibody (4D11)
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FIGURE: 1 / 2
Nestin Antibody (MA5-47329) in ICC/IF
Immunofluorescent analysis of Nestin in cortical neuron-glial cell culture from E20 rat. Samples were stained with Nestin monoclonal antibody (Product # MA5-47428) using a dilution of 1:500 (red). Costained with chicken pAb to MAP2 dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-47329
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4D11
Immunogen
Recombinant construct, amino acids 317-630 of the human protein expressed in and purified from E. coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Protein G
Storage buffer
PBS with 50% glycerol
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ21841; intermediate filament protein; intermediate filament-associated protein RC2; nestin (128 could be 161); unnamed protein product
Gene Aliases: ESTM46; Ifaprc2; Marc2; Nbla00170; RC2
UniProt ID: (Human) P48681, (Mouse) Q6P5H2
Entrez Gene ID: (Human) 10763, (Rat) 25491, (Mouse) 18008
G2/M transition of mitotic cell cycle
central nervous system development
brain development
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
negative regulation of protein binding
negative regulation of catalytic activity
stem cell proliferation
positive regulation of neural precursor cell proliferation
nervous system development
response to xenobiotic stimulus
response to ionizing radiation
neuron differentiation
response to nutrient levels
negative regulation of neuron apoptotic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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