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Invitrogen
PGP9.5 Monoclonal Antibody (BH7)
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FIGURE: 1 / 3
PGP9.5 Antibody (MA5-47376) in ICC/IF
Immunofluorescent analysis of PGP9.5 in cortical neuron-glial culture from E20 rat. Samples were stained with PGP9.5 monoclonal antibody (Product # MA5-47475) using a dilution of 1:5,000 (green). Costained with chicken pAb to GFAP dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. The PGP9.5 antibody stains cell bodies and dendrites of neurons, while the GFAP antibody labels astrocytes.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-47376
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Horse,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
BH7
Immunogen
Recombinant full length human UCHL1 expressed in and purified from E. coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Protein G
Storage buffer
PBS with 50% glycerol
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis luminal protein 117; epididymis secretory protein Li 53; gracile axonal dystrophy; neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PGP 9.5; PGP9.5; protein gene product 9.5; ubiquitin C-terminal hydrolase 1; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase L1; ubiquitin thioesterase L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1; UCH-L1; UCHL; unnamed protein product
Gene Aliases: gad; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; SPG79A; UCH-L1; UCHL-1
UniProt ID: (Pig) Q6SEG5, (Human) P09936, (Horse) Q9GM50, (Bovine) P23356, (Mouse) Q9R0P9, (Rat) Q00981
Entrez Gene ID: (Pig) 396637, (Human) 7345, (Horse) 100033838, (Bovine) 514394, (Mouse) 22223, (Rat) 29545
response to ischemia
ubiquitin-dependent protein catabolic process
axon target recognition
adult walking behavior
cell proliferation
axonal transport of mitochondrion
cellular response to drug
eating behavior
negative regulation of MAP kinase activity
muscle fiber development
neuromuscular process
regulation of macroautophagy
protein deubiquitination
protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of MAPK cascade
positive regulation of glycolytic process
axonogenesis
muscle cell development
cellular response to xenobiotic stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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