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Invitrogen
GRB2 Monoclonal Antibody (M331)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-47669
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M331
Immunogen
Clone (M331) was generated from a full length recombinant rat Grb2 protein. This sequence has high homology to human and mouse Grb2.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.125 mg/mL
Amount
12.5 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects a 24 kDa* protein corresponding to the molecular mass of Grb2 on SDS-PAGE immunoblots of rat PC12.
Target Information
GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: abundant SRC homology; adapter protein GRB2; adaptor protein; ASH protein; epidermal growth factor receptor-binding protein GRB2; epididymis secretory sperm binding protein; GRB2; growth factor receptor-bound protein 2-like; growth factor receptor-bound protein 3; HT027; OTTHUMP00000166097; OTTHUMP00000166098; protein Ash; SH2/SH3 adapter GRB2; unnamed protein product
Gene Aliases: ASH; Ash-psi; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; NCKAP2
UniProt ID: (Human) P62993, (Mouse) Q60631, (Rat) P62994
Entrez Gene ID: (Human) 2885, (Mouse) 14784, (Rat) 81504
phosphotyrosine binding
RNA binding
transmembrane receptor protein tyrosine kinase adaptor activity
guanyl-nucleotide exchange factor adaptor activity
epidermal growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
SH3 domain binding
protein kinase binding
protein phosphatase binding
protein domain specific binding
protein binding, bridging
identical protein binding
insulin receptor substrate binding
ephrin receptor binding
enzyme binding
macromolecular complex binding
phosphoprotein binding
binding, bridging
signal transduction
epidermal growth factor receptor signaling pathway
Ras protein signal transduction
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
Schwann cell development
actin cytoskeleton organization
cell differentiation
positive regulation of actin filament polymerization
T cell costimulation
receptor internalization
ERBB2-ERBB3 signaling pathway
T cell activation
natural killer cell mediated cytotoxicity
myelination
signal transduction in response to DNA damage
regulation of MAPK cascade
negative regulation of natural killer cell mediated cytotoxicity
insulin-like growth factor receptor signaling pathway
anatomical structure formation involved in morphogenesis
B cell receptor signaling pathway
branching involved in labyrinthine layer morphogenesis
cellular response to ionizing radiation
positive regulation of reactive oxygen species metabolic process
positive regulation of Rac protein signal transduction
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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