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Invitrogen
Talin Monoclonal Antibody (8D4)
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FIGURE: 1 / 1
Talin Antibody (MA5-47740) in WB
Western blot of Talin in rat PC12 cells stimulated with Calyculin A (100 nM) for 30 minutes (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse Talin monoclonal antibody (Product # MA5-47740) (lanes 1 & 2) or rabbit polyclonal antibody anti-Talin (Ser425) (lanes 3 & 4).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-47740
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8D4
Immunogen
Clone 8D4 was generated from purified chicken gizzard talin. The antibody recognizes an epitope within the rod domain of talin from many species, including human, rat, mouse, chicken, and frog.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells.
Target Information
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytoskeletal protein; KIAA0320; unnamed protein product
Gene Aliases: 5730421P04Rik; ILWEQ; mKIAA0320; RGD1565416; talin-1; talin-2; TLN
UniProt ID: (Chicken) P54939, (Human) Q9Y490, (Human) Q9Y4G6, (Mouse) P26039, (Mouse) Q71LX4
Entrez Gene ID: (Chicken) 395194, (Human) 7094, (Chicken) 415374, (Human) 83660, (Rat) 313494, (Mouse) 21894, (Rat) 315776, (Mouse) 70549
structural constituent of cytoskeleton
protein binding
clathrin binding
clathrin light chain binding
phosphatidylinositol binding
clathrin adaptor activity
phosphatidylinositol-3,4-bisphosphate binding
actin filament binding
phosphatidylinositol-3,5-bisphosphate binding
phosphatidylserine binding
actin binding
integrin binding
vinculin binding
LIM domain binding
cadherin binding
structural molecule activity
non-motor actin binding protein
endocytosis
actin filament organization
cell adhesion
positive regulation of receptor-mediated endocytosis
clathrin coat assembly
cell-cell junction assembly
integrin-mediated signaling pathway
actin cytoskeleton organization
integrin activation
cortical microtubule organization
regulation of focal adhesion assembly
platelet aggregation
cell-cell adhesion
presynaptic actin cytoskeleton organization
cell-substrate junction assembly
biological_process
cortical actin cytoskeleton organization
cell junction assembly
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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