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Invitrogen
HP1 alpha Chimeric Recombinant Rabbit Monoclonal Antibody (RAB-C133)
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FIGURE: 1 / 3
HP1 alpha Chimeric Antibody (MA5-48329) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of HP1 alpha in HeLa cells using HP1 alpha Chimeric Monoclonal Antibody (Product # MA5-48329). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton and stained with HP1 alpha Chimeric Monoclonal Antibody at a dilution of 1:100 for 1h followed by Alexa Fluor 488 secondary antibody (1:1,000 dilution), showing nuclear staining. The nuclear stai... View More
Product Details
MA5-48329
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RAB-C133
Immunogen
This antibody was obtained by recombinant antibody (rAb) phage display recognizing CBX5 protein under non-denaturing conditions.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Specificity: This antibody recognizes CBX5 (Chromobox protein homolog 5). It binds to a folded domain, amino acids 18-75. CBX5 is a component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 25 kda chromosomal autoantigen; Antigen p25; CBX5; Cbx5 antibody; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; epididymis luminal protein 25; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HGNC:1555; HP-1 alpha antibody; HP1 alpha; HP1 alpha homolog; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha; unnamed protein product
Gene Aliases: CBX5; HEL25; HP1; HP1A; HP1alpha
UniProt ID: (Human) P45973
Entrez Gene ID: (Human) 23468
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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