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Invitrogen
CYP2E1 Monoclonal Antibody (2C7G1)
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FIGURE: 1 / 1
CYP2E1 Antibody (MA5-49163) in WB
Western blot analysis of Cytochrome P450 2E1/CYP2E1 using Cytochrome P450 2E1/CYP2E1 antibody (Product # MA5-49163). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human HCCP tissue lysates; Lane 2: rat liver tissue lysates; Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the mem... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-49163
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2C7G1
Immunogen
E.coli-derived human Cytochrome P450 2E1/CYP2E1 recombinant protein (Position: H355-S493).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HCCP tissue, rat liver tissue, mouse liver tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4-nitrophenol 2-hydroxylase; CP2E1; CYPIIE1; cytochrome P450 subfamily 2e1 (ethanol-inducible); cytochrome P450, 2e1, ethanol inducible; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily 2E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; cytochrome P450-ALC; cytochrome P450-J; cytochrome P450A; cytochrome P450j; cytochrome P450RLM6; flavoprotein-linked monooxygenase; microsomal monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; mouse Cyp2e1; P450-J; P450RLM6; unnamed protein product; xenobiotic monooxygenase
Gene Aliases: CPE1; CYP2E; CYPIIE1; P450-J; P450C2E
UniProt ID: (Human) P05181, (Mouse) Q05421, (Rat) P05182
Entrez Gene ID: (Human) 1571, (Mouse) 13106, (Rat) 25086
monooxygenase activity
iron ion binding
arachidonic acid epoxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
4-nitrophenol 2-monooxygenase activity
oxygen binding
enzyme binding
heme binding
Hsp70 protein binding
Hsp90 protein binding
long-chain fatty acid omega-1 hydroxylase activity
fatty acid omega-1 hydroxylase activity
long-chain fatty acid metabolic process
lipid hydroxylation
fatty acid metabolic process
triglyceride metabolic process
xenobiotic metabolic process
steroid metabolic process
response to bacterium
monoterpenoid metabolic process
4-nitrophenol metabolic process
epoxygenase P450 pathway
response to xenobiotic stimulus
response to ozone
response to ethanol
response to 3-methylcholanthrene
carbon tetrachloride metabolic process
benzene metabolic process
halogenated hydrocarbon metabolic process
long-chain fatty acid biosynthetic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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