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Invitrogen
GPX4 Monoclonal Antibody (6I4E7)
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GPX4 Antibody (MA5-49216) in WB
Western blot analysis of Glutathione Peroxidase 4/GPX4 using Glutathione Peroxidase 4/GPX4 antibody (Product # MA5-49216). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates; Lane 2: human CACO-2 whole cell lysates; Lane 3: human 293T whole cell lysates; Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocel... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-49216
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
6I4E7
Immunogen
E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human CACO-2 whole cell, human 293T whole cell, human K562 whole cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; HGNC:4556; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; selenoprotein GPX4; sperm nucleus glutathione peroxidase
Gene Aliases: GPx-4; GSHPx-4; MCSP; PHGPx; SMDS; snGPx; snPHGPx
UniProt ID: (Human) P36969
Entrez Gene ID: (Human) 2879
phospholipid metabolic process
response to oxidative stress
spermatogenesis
arachidonic acid metabolic process
lipoxygenase pathway
response to estradiol
cellular response to oxidative stress
long-chain fatty acid biosynthetic process
protein polymerization
cellular oxidant detoxification
negative regulation of ferroptosis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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