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Invitrogen
ATP Citrate Lyase Monoclonal Antibody (5I2)
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FIGURE: 1 / 8
ATP Citrate Lyase Antibody (MA5-49221) in ICC/IF
Immunocytochemistry analysis of ATP Citrate Lyase in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in ATP Citrate Lyase Monoclonal antibody (Product # MA5-49221) using a dilution of 2 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 di... View More
Product Details
MA5-49221
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
5I2
Immunogen
E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.
Positive Control - WB: human placenta tissue, U-87MG whole cell, HEK293 whole cell, Caco-2 whole cell, HL-60 whole cell, Raji whole cell, THP-1 whole cell, PANC-1 whole cell, rat lung tissue, rat testicular tissue, rat kidney tissue, rat brain tissue, mouse lung tissue, mouse testicular tissue, mouse kidney tissue. IHC: human pancreatic cancer tissue, human testis cancer tissue, mouse pancreas tissue, rat pancreas tissue. ICC/IF: MCF7 cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACL; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; Citrate cleavage enzyme; unnamed protein product
Gene Aliases: A730098H14Rik; ACL; ACLY; ATPCL; AW538652; CLATP
UniProt ID: (Human) P53396, (Mouse) Q91V92, (Rat) P16638
Entrez Gene ID: (Human) 47, (Mouse) 104112, (Rat) 24159
nucleotide binding
catalytic activity
ATP citrate synthase activity
protein binding
ATP binding
transferase activity
metal ion binding
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
succinate-CoA ligase (ADP-forming) activity
cofactor binding
lyase activity
transferase
acetyl-CoA biosynthetic process
citrate metabolic process
oxaloacetate metabolic process
lipid metabolic process
fatty acid biosynthetic process
cholesterol biosynthetic process
lipid biosynthetic process
coenzyme A metabolic process
negative regulation of ferroptosis
acetyl-CoA metabolic process
metabolic process
cellular carbohydrate metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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