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Invitrogen
PCBP2 Monoclonal Antibody (4B9C7)
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FIGURE: 1 / 4
PCBP2 Antibody (MA5-49224) in ICC/IF
Immunocytochemistry analysis of PCBP2 in Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PCBP2 Monoclonal antibody (Product # MA5-49224) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
MA5-49224
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
4B9C7
Immunogen
E.coli-derived human PCBP2/hnRNP E2 recombinant protein (Position: Q197-K276).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human T-47D whole cell, human HL-60 whole cell, human A549 whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue. ICC/IF: Caco-2 cell. Flow: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha CP2; Alpha-CP2; CBP; CTBP; epididymis secretory sperm binding protein; Heterogeneous nuclear ribonucleoprotein E2; heterogeneous nuclear ribonucleoprotein X; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; hnRNP X; MGC110998; Poly(rC)-binding protein 2; Putative heterogeneous nuclear ribonucleoprotein X; rCbinding protein 2; unnamed protein product
Gene Aliases: alphaCP-2; AW412548; Cbp; hnRNP-E2; HNRNPE2; Hnrnpx; HNRPE2; Hnrpx; PCBP2
UniProt ID: (Human) Q15366, (Mouse) Q61990
Entrez Gene ID: (Human) 5094, (Rat) 363005, (Mouse) 18521
immune system process
regulation of transcription from RNA polymerase II promoter
mRNA metabolic process
viral RNA genome replication
proteasome-mediated ubiquitin-dependent protein catabolic process
innate immune response
negative regulation of defense response to virus
defense response to virus
IRES-dependent viral translational initiation
chromatin looping
cGAS/STING signaling pathway
negative regulation of cGAS/STING signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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