Search
Invitrogen
CKAP5 Monoclonal Antibody (3C13)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 8
CKAP5 Antibody (MA5-49287) in ICC/IF
Immunocytochemistry analysis of CKAP5 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in CKAP5 Monoclonal antibody (Product # MA5-49287) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-49287
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3C13
Immunogen
E.coli-derived human ch TOG/CKAP5 recombinant protein (Position: M1-L221).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human U-87MG whole cell, human COLO-320 whole cell, human SW620 whole cell, human HEPG2 whole cell, human CACO-2 whole cell, rat brain tissue, mouse brain tissue. IHC: human liver cancer tissue, human rectal cancer tissue, human renal cancer tissue, rat brain tissue. ICC/IF: Hela cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Colonic and hepatic tumor over-expressed protein (ch-TOG) belongs to the TOG/XMAP215/Dis1 evolutionarily ancient family of microtubule-associated proteins with isoforms found in all major kingdoms of eukaryotes. This protein is required for the assembly and function of the meiotic or mitotic spindles during cell division and is localized to microtubule-organizing centers (MTOCs) during interphase or to the spindle poles and centrosomes during mitosis or cytokinesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: ch-TOGp; colonic and hepatic tumor over-expressed gene protein; colonic and hepatic tumor overexpressed gene protein; unnamed protein product
Gene Aliases: 3110043H24Rik; 4930432B04Rik; ch-TOG; CHTOG; D730027C18Rik; mKIAA0097; MSPS; TOG; TOGp
UniProt ID: (Human) Q14008, (Mouse) A2AGT5
Entrez Gene ID: (Human) 9793, (Mouse) 75786, (Rat) 311191
mitotic cell cycle
microtubule depolymerization
spindle organization
mitotic spindle organization
chromosome segregation
centrosome cycle
establishment or maintenance of microtubule cytoskeleton polarity
protein-containing complex subunit organization
microtubule polymerization
RNA transport
centrosome duplication
positive regulation of microtubule nucleation
cytoplasmic translation
locomotory behavior
visual learning
mRNA localization resulting in posttranscriptional regulation of gene expression
ribonucleoprotein complex assembly
central nervous system myelin formation
positive regulation of endocytosis
habituation
excitatory postsynaptic potential
long-term synaptic potentiation
dendritic spine maintenance
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support