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Invitrogen
Caspase 3 Recombinant Rabbit Monoclonal Antibody (14C1)
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FIGURE: 1 / 3
Caspase 3 Antibody (MA5-50123) in IHC (P)
Immunohistochemistry analysis with a Caspase 3 monoclonal antibody (Product # MA5-50123) using a dilution of 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-50123
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
14C1
Immunogen
A synthesized peptide derived from Human CASP3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.2 mg/mL
Amount
120 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apopain; Apopain precursor; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase3; CPP-32; cysteine protease CPP32; ICE3; PARP cleavage protease; procaspase3; protein Yama; SREBP cleavage activity 1; unnamed protein product
Gene Aliases: CPP32; CPP32B; SCA-1
UniProt ID: (Human) P42574
Entrez Gene ID: (Human) 836
protease binding
endopeptidase activity
aspartic-type endopeptidase activity
cysteine-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
death receptor binding
protein binding
enzyme activator activity
peptidase activity
cysteine-type peptidase activity
phospholipase A2 activator activity
macromolecular complex binding
luteolysis
response to hypoxia
negative regulation of cytokine production
proteolysis
apoptotic process
axonal fasciculation
learning or memory
intrinsic apoptotic signaling pathway in response to osmotic stress
response to xenobiotic stimulus
response to glucose
response to metal ion
response to X-ray
regulation of macroautophagy
protein processing
hippocampus development
protein catabolic process
neuron differentiation
keratinocyte differentiation
erythrocyte differentiation
platelet formation
regulation of protein stability
response to cobalt ion
response to estradiol
response to lipopolysaccharide
regulation of protein localization
glial cell apoptotic process
response to tumor necrosis factor
response to nicotine
intracellular signal transduction
swimming behavior
response to hydrogen peroxide
positive regulation of apoptotic process
response to amino acid
regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
response to ethanol
neurotrophin TRK receptor signaling pathway
striated muscle cell differentiation
response to glucocorticoid
protein maturation
pyroptosis
leukocyte apoptotic process
response to anesthetic
cellular response to staurosporine
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
regulation of synaptic vesicle cycle
synapse pruning
positive regulation of pyroptotic inflammatory response
positive regulation of beta-amyloid formation
positive regulation of apoptotic DNA fragmentation
response to insulin-like growth factor stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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