Immunohistochemistry (Paraffin) analysis of Dystrophin in Human Cerebrum Tissue. Samples were incubated in Dystrophin monoclonal antibody (Product # MA5-50847) using a dilution of 30 µg/mL followed by HRP-Linked Caprine Anti-Mouse IgG at a dilution of 2 µg/mL and DAB staining.

Dystrophin Antibody in Western Blot (WB)

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Immunogen Image Dystrophin Monoclonal Antibody (C8)

Product Details

MA5-50847

Applications

Tested Dilution

Publications

Western Blot (WB)

0.01-3 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

5-30 µg/mL

Product Specifications

Species Reactivity

Human, Pig, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Recombinant Human DMD protein, Ile253-Lys597 (Accession #P11532)

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

200 µg

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.05% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Ambient (domestic); Wet ice (international)

AB_3094005

Target Information

Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immunohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: apodystrophin-3; apodystrophin-I; DMD; Dpm234; Duchenne muscular dystrophy (DMD); dystrophin; dystrophin, muscular dystrophy; dystrophin-related; GS1-19O24.1; Muscular dystrophy Duchenne and Becker types; mutant dystrophin; OTTHUMP00000215592; unnamed protein product

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Gene Aliases: BMD; CMD3B; DNADMD1; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; MRX85

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UniProt ID: (Human) P11532, (Pig) Q5GN48, (Rat) P11530

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Entrez Gene ID: (Human) 1756, (Pig) 497636, (Rat) 24907

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Function(s)

dystroglycan binding actin binding integrin binding structural constituent of cytoskeleton protein binding lamin binding zinc ion binding structural constituent of muscle myosin binding vinculin binding PDZ domain binding macromolecular complex binding nitric-oxide synthase binding ubiquitin-protein ligase

Process(es)

positive regulation of cell-matrix adhesion regulation of heart rate muscle contraction cytoskeleton organization muscle organ development skeletal muscle tissue development protein localization positive regulation of cell proliferation regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion positive regulation of neuron projection development regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion regulation of skeletal muscle contraction response to denervation involved in regulation of muscle adaptation cerebral cortex development cell differentiation neuron differentiation maintenance of blood-brain barrier response to muscle stretch muscle cell differentiation camera-type eye development peptide biosynthetic process skeletal muscle tissue regeneration regulation of system process motile cilium assembly negative regulation of neuron differentiation positive regulation of neuron differentiation muscle cell cellular homeostasis neuron development neuron projection morphogenesis muscle cell development cardiac muscle contraction protein-containing complex assembly cardiac muscle cell action potential regulation of muscle system process regulation of cellular response to growth factor stimulus synaptic signaling regulation of sodium ion transmembrane transport regulation of calcium ion transmembrane transport regulation of transcription, DNA-templated apoptotic process striated muscle contraction inflammatory response establishment of blood-nerve barrier determination of adult lifespan response to xenobiotic stimulus gene expression regulation of gene expression myotube cell development olfactory nerve structural organization multicellular organism growth regulation of membrane potential nucleus localization striated muscle cell development limb development bone development establishment of glial blood-brain barrier connective tissue development negative regulation of ERK1 and ERK2 cascade walking behavior reactive oxygen species biosynthetic process

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Dystrophin Monoclonal Antibody (C8)

Dystrophin Antibody (MA5-50847) in IHC (P)

Product Details

MA5-50847

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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