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Invitrogen
Nephrin Monoclonal Antibody (C14)
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FIGURE: 1 / 2
Nephrin Antibody (MA5-50997) in WB
Western blot analysis of Nephrin in various lysates. Samples were incubated with Nephrin monoclonal antibody (Product # MA5-50997) using a dilution of 2 µg/mL followed by HRP-Linked Caprine Anti-Mouse IgG secondary antibody at a dilution of 0.2 µg/mL. Lane 1: Human Serum; Lane 2: Rat Kidney lysate; Lane 3: Mouse Kidney lysate; Lane 4: Porcine Kidney lysate; Lane 5: Rat Liver lysate; Lane 6: 293T cell lysate; Lane 7: HepG2 cell lysate.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-50997
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
C14
Immunogen
Recombinant Human NPHN protein, Arg1160-Val1241 (Accession #O60500)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.05% Proclin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling. Nephrin seems to play a role in the development or function of the kidney glomerular filtration barrier and it may anchor the podocyte slit diaphragm to the actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: immunoglobulin superfamily member; nephrin; nephrin 1; nephrosis 1 homolog, nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHS1; NPHS1 nephrin; NPHS1, nephrin; protein nephrosis 1; renal glomerulus-specific cell adhesion receptor
Gene Aliases: CNF; nephrin; NephrinB; NPHN
UniProt ID: (Human) O60500, (Rat) Q9R044, (Mouse) Q9QZS7
Entrez Gene ID: (Human) 4868, (Rat) 64563, (Mouse) 54631
skeletal muscle tissue development
myoblast fusion
glomerular basement membrane development
protein localization to synapse
slit diaphragm assembly
glomerular visceral epithelial cell development
cell-cell adhesion
MAPK cascade
glomerular filtration
cell adhesion
JNK cascade
gene expression
positive regulation of actin filament polymerization
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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