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SHH Monoclonal Antibody (D6)
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FIGURE: 1 / 7
SHH Antibody (MA5-51177) in IHC (P)
Immunohistochemistry (Paraffin) analysis of SHH in human small intestine tissue using SHH Monoclonal Antibody (D6) (Product # MA5-51177) (40 µg/mL) and using PBS intead of the primary antibody as a control. The sample was then stained with HRP-Goat anti-Mouse IgG polyclonal secondary antibody (2 µg/mL). DAB staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-51177
Product Specifications
Species Reactivity
Human,
Pig
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
D6
Immunogen
Recombinant Human SHH protein, Pro36-Ala193 (Accession #Q15465)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.05% Proclin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SHH signaling molecule; shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; sonic hedgehog homolog
Gene Aliases: HHG1; HLP3; HPE3; MCOPCB5; ShhNC; SMMCI; TPT; TPTPS
UniProt ID: (Human) Q15465
Entrez Gene ID: (Pig) 100512749, (Human) 6469
negative regulation of transcription from RNA polymerase II promoter
patterning of blood vessels
vasculogenesis
metanephros development
branching involved in ureteric bud morphogenesis
cell fate specification
neural crest cell migration
heart looping
lymphoid progenitor cell differentiation
determination of left/right asymmetry in lateral mesoderm
smoothened signaling pathway
cell-cell signaling
pattern specification process
neuroblast proliferation
axon guidance
central nervous system development
ventral midline development
heart development
androgen metabolic process
positive regulation of cell proliferation
embryonic pattern specification
tissue development
polarity specification of anterior/posterior axis
dorsal/ventral pattern formation
regulation of gene expression
negative regulation of gene expression
intein-mediated protein splicing
protein autoprocessing
cerebellar granule cell precursor proliferation
regulation of proteolysis
neuron differentiation
lung development
embryonic limb morphogenesis
negative regulation of cell migration
male genitalia development
prostate gland development
forebrain development
midbrain development
hindbrain development
T cell differentiation in thymus
positive regulation of T cell differentiation in thymus
positive regulation of immature T cell proliferation in thymus
tube development
regulation of cell proliferation
regulation of odontogenesis
embryonic digit morphogenesis
negative regulation of apoptotic process
CD4-positive or CD8-positive, alpha-beta T cell lineage commitment
positive thymic T cell selection
negative thymic T cell selection
cell fate commitment
negative regulation of cell differentiation
positive regulation of smoothened signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of alpha-beta T cell differentiation
cell development
animal organ development
thymus development
neuron fate commitment
oligodendrocyte differentiation
smooth muscle tissue development
branching morphogenesis of an epithelial tube
stem cell development
regulation of developmental process
protein maturation
positive regulation of cell division
epithelial-mesenchymal cell signaling
somite development
positive regulation of sclerotome development
dopaminergic neuron differentiation
metanephric mesenchymal cell proliferation involved in metanephros development
metanephric collecting duct development
negative regulation of cholesterol efflux
apoptotic signaling pathway
regulation of nodal signaling pathway
regulation of protein localization to nucleus
negative regulation of dopaminergic neuron differentiation
negative regulation of ureter smooth muscle cell differentiation
positive regulation of ureter smooth muscle cell differentiation
negative regulation of kidney smooth muscle cell differentiation
positive regulation of kidney smooth muscle cell differentiation
positive regulation of mesenchymal cell proliferation involved in ureter development
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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