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Invitrogen
HK2 Recombinant Rabbit Monoclonal Antibody (5Z8C3)
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FIGURE: 1 / 2
HK2 Antibody (MA5-51399) in WB
Western blot analysis of HK2 in various lysates. Samples were incubated with HK2 Recombinant monoclonal antibody (Product # MA5-51399) using a dilution of 1:1,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-51399
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5Z8C3
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2).
Conjugate
Form
Liquid
Concentration
2.23 mg/mL
Amount
223 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR
Target Information
In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686M1669; Hexokinase; Hexokinase type II; Hexokinase-2; hexokinase-2 muscle; hexokinase-2, muscle; Hexokinase-B; HK II; Muscle form hexokinase; OTTHUMP00000202738; unnamed protein product
Gene Aliases: AI642394; HK2; HKII; HXK2
UniProt ID: (Human) P52789, (Mouse) O08528, (Rat) P27881
Entrez Gene ID: (Human) 3099, (Mouse) 15277, (Rat) 25059
response to hypoxia
cellular glucose homeostasis
response to ischemia
carbohydrate metabolic process
fructose 6-phosphate metabolic process
glucose metabolic process
glycolytic process
lactation
apoptotic mitochondrial changes
hexose metabolic process
organophosphate metabolic process
negative regulation of mitochondrial membrane permeability
positive regulation of angiogenesis
regulation of glucose import
carbohydrate phosphorylation
glucose 6-phosphate metabolic process
canonical glycolysis
establishment of protein localization to mitochondrion
maintenance of protein location in mitochondrion
carbohydrate derivative metabolic process
positive regulation of type 2 mitophagy
cellular response to leukemia inhibitory factor
negative regulation of reactive oxygen species metabolic process
metabolic process
phosphorylation
glucose catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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