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Invitrogen

HIF1A VHH-8His-Cys-tag Recombinant Alpaca Monoclonal Antibody (SAA1239)

View all (81) HIF1A antibodies

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Product Details

MA5-54597

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Alpaca / VHH

Expression System

CHO cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SAA1239

Immunogen

Recombinant Human HIF1A.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Metal-chelate chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_3666847

Product Specific Information

Compatible with Direct ELISA

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ARNT interacting protein; ARNT2; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; hif 1; hif 1a; HIF-1alpha 786; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8; transcription factor; transcription factor; hypoxia-inducible factor 1a; unnamed protein product

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Gene Aliases: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8

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UniProt ID: (Human) Q16665

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Entrez Gene ID: (Human) 3091

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ligand-dependent nuclear receptor binding enzyme binding protein kinase binding protein domain specific binding ubiquitin protein ligase binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding transcription regulator activator activity
Process(es)
response to reactive oxygen species response to hypoxia cellular glucose homeostasis epithelial to mesenchymal transition positive regulation of endothelial cell proliferation connective tissue replacement involved in inflammatory response wound healing regulation of glycolytic process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter signal transduction response to iron ion regulation of gene expression vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration axonal transport of mitochondrion negative regulation of ossification positive regulation of vascular endothelial growth factor receptor signaling pathway oxygen homeostasis positive regulation of chemokine production regulation of transforming growth factor beta2 production collagen metabolic process cellular response to oxidative stress positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of angiogenesis positive regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone biosynthetic process elastin metabolic process positive regulation of chemokine-mediated signaling pathway cellular response to interleukin-1 cellular response to hypoxia cellular response to virus positive regulation of cytokine production involved in inflammatory response negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of reactive oxygen species biosynthetic process regulation of protein neddylation
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