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Invitrogen
PDK1 Recombinant Rabbit Monoclonal Antibody (9A7X4)
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FIGURE: 1 / 1
PDK1 Antibody (MA5-55304) in WB
Western Blot analysis of various lysates using PDK1 Recombinant Monoclonal Antibody (Product # MA5-55304) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-55304
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9A7X4
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PDK1/PDHK1.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
Target Information
PDK1 (pyruvate dehydrogenase kinase, isozyme 1) gene, located on chromosome 2q31.1, encodes a member of the pyruvate dehydrogenase kinase family. This kinase plays a crucial role in the regulation of glucose metabolism by phosphorylating and inactivating the pyruvate dehydrogenase complex (PDC), which converts pyruvate to acetyl-CoA, linking glycolysis to the Krebs cycle. Structurally, the PDK1 protein contains a kinase domain that is essential for its enzymatic activity. PDK1 acts as a gatekeeper of glucose oxidation and is tightly regulated by various metabolic signals, including insulin and hypoxia. Dysregulation of PDK1 activity has been implicated in metabolic disorders such as diabetes and cancer, where it contributes to altered cellular energy homeostasis and metabolic reprogramming.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; isoenzyme 1; kinase isoenzyme 1; lipoamide; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; PDK1; pyruvate dehydrogenase; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1; unnamed protein product
Gene Aliases: B830012B01; D530020C15Rik; PDHK1; PDK1
UniProt ID: (Human) Q15118, (Mouse) Q8BFP9
Entrez Gene ID: (Human) 5163, (Mouse) 228026
nucleotide binding
protein kinase activity
pyruvate dehydrogenase (acetyl-transferring) kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein serine/threonine kinase activity
protein complex binding
protein homodimerization activity
protein heterodimerization activity
non-receptor serine/threonine protein kinase
glucose metabolic process
cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
regulation of acetyl-CoA biosynthetic process from pyruvate
regulation of glucose metabolic process
cellular response to stress
hypoxia-inducible factor-1alpha signaling pathway
carbohydrate metabolic process
protein phosphorylation
cell surface receptor signaling pathway
phosphorylation
peptidyl-serine phosphorylation
intracellular signal transduction
mitophagy in response to mitochondrial depolarization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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