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Invitrogen
MRPS31 Recombinant Rabbit Monoclonal Antibody (3J3T5)
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FIGURE: 1 / 2
MRPS31 Antibody (MA5-55493) in WB
Western Blot analysis of various lysates using MRPS31 Recombinant Monoclonal Antibody (Product # MA5-55493) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-55493
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3J3T5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 296-395 of human MRPS31.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
Product Specific Information
Immunogen Sequence: NGFEELIQWT KEGKLWEFPI NNEAGFDDDG SEFHEHIFLE KHLESFPKQG PIRHFMELVT CGLSKNPYLS VKQKVEHIEW FRNYFNEKKD ILKESNIQFN
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 28S ribosomal protein S31, mitochondrial; Imogen 38; islet mitochondrial antigen, 38 kD; mitochondrial small ribosomal subunit protein mS31; MRP-S31; S31mt; Small ribosomal subunit protein mS31; unnamed protein product
Gene Aliases: 1500002D03Rik; AA960481; Imogen38; Imogen44; IMOGN38; MRP-S31; MRPS31; mS31; S31mt
UniProt ID: (Human) Q92665, (Rat) B0BN56, (Mouse) Q61733
Entrez Gene ID: (Human) 10240, (Rat) 290850, (Mouse) 57312
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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