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Invitrogen
NSUN2 Recombinant Rabbit Monoclonal Antibody (PSH05-05)
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FIGURE: 1 / 5
NSUN2 Antibody (MA5-56501) in ICC/IF
Immunocytochemistry analysis of NSUN2 with HeLa cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with NSUN2 Recombinant Monoclonal Antibody (Product # MA5-56501)at a dilution of 1:250 in 1% BSA in PBST overnight at 4 &(8)451;. Followed by Goat Anti-Rabbit IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).
Product Details
MA5-56501
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH05-05
Immunogen
Recombinant protein within human NSUN2 aa 1-767.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA). Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Mutations of NSUN2 result in autosomal recessive 5 mental retardation (MRT5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-methycytoisine methyltransferase; hTrm4; Misu; mRNA cytosine C(5)-methyltransferase; Myc-induced SUN domain containing protein; Myc-induced SUN domain-containing protein; Myc-induced SUN-domain-containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family member 2; NOL1/NOP2/Sun domain family, member 2; NOP2/Sun domain family, member 2; NOP2/Sun RNA methyltransferase family member 2; RNA cytosine C(5)-methyltransferase NSUN2; Substrate of AIM1/Aurora kinase B; tRNA (cytosine(34)-C(5))-methyltransferase; tRNA (cytosine-5-)-methyltransferase NSUN2; tRNA cytosine C(5)-methyltransferase; tRNA methyltransferase 4 homolog; unnamed protein product
Gene Aliases: D13Wsu123e; MISU; MRT5; NSUN2; SAKI; TRM4
UniProt ID: (Human) Q08J23, (Mouse) Q1HFZ0
Entrez Gene ID: (Human) 54888, (Mouse) 28114
tRNA binding
RNA binding
protein binding
methyltransferase activity
RNA methyltransferase activity
S-adenosylmethionine-dependent methyltransferase activity
tRNA (cytosine-5-)-methyltransferase activity
transferase activity
mRNA (cytidine-5-)-methyltransferase activity
poly(A) RNA binding
RNA methyltransferase
RNA methylation
in utero embryonic development
mRNA processing
tRNA modification
spermatogenesis
spermatid development
tRNA processing
regulation of mRNA export from nucleus
cell differentiation
tRNA methylation
methylation
meiotic cell cycle checkpoint
tRNA stabilization
hair follicle maturation
cell division
regulation of stem cell differentiation
cell cycle
mitotic nuclear division
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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