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Invitrogen
MELK Recombinant Rabbit Monoclonal Antibody (24GB3530)
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FIGURE: 1 / 3
MELK Antibody (MA5-57071) in ICC/IF
Immunocytochemical staining of MELK in HepG2 cells using MELK Recombinant Monoclonal Antibody (Product # MA5-57071) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; Maternal embryonic leucine zipper kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57071
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB3530
Immunogen
A synthesized peptide derived from human MELK (aa 1-36)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.6 mg/mL
Amount
60 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
MELK is a serine/threonione kinase which is related to the AMPK family that has been linked to the cell cycle. MELK has also been shown to be overexpressed in cancer and has been identified has a potential target for cancer therapeutics. MELK has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: KIAA0175; OTTHUMP00000046113; pEg3 kinase; protein kinase Eg3; protein kinase PK38; RP11-8N6.1; tyrosine-protein kinase MELK; unnamed protein product
Gene Aliases: HPK38; mKIAA0175; MPK38
UniProt ID: (Human) Q14680, (Mouse) Q61846
Entrez Gene ID: (Human) 9833, (Rat) 362510, (Mouse) 17279
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