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          • Primary Antibodies ›
          • Nidogen Antibodies

          Invitrogen

          Nidogen Recombinant Rabbit Monoclonal Antibody (24GB4995)

          View all (27) Nidogen antibodies

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          Cite Nidogen Recombinant Rabbit Monoclonal Antibody (24GB4995)

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          • Antibody Testing Data (2)
          Nidogen Antibody in Western Blot (WB)
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          Nidogen Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Nidogen Antibody (MA5-57119) in WB

          Western blot analysis of Nidogen. Total cell lysates (30 μg) from C2C12, H9c2, HAP-1, HeLa, HepG2, and HT-1080 cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nidogen Recombinant Monoclonal Antibody (Product # MA5-57119) at a 1:5,000 dilution and HRP-conjugated goat anti-rabbit secondary antibody at a 1:20,000 dilution respectively. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Nidogen Antibody in Western Blot (WB)
          Nidogen Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Nidogen Recombinant Rabbit Monoclonal Antibody (24GB4995)

          Product Details

          MA5-57119

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:5,000
          -

          Flow Cytometry (Flow)

          1:2,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293F

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          24GB4995

          Immunogen

          Recombinant protein of human Entactin/NID (aa 844-1245)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.7 mg/mL

          Amount

          70 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Dry ice (international)

          RRID

          AB_3680145

          Target Information

          This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: enactin; entactin; entactin 1; epididymis secretory sperm binding protein; NID 1; NID-1; Nidogen (enactin); nidogen (entactin); Nidogen precursor (Entactin); Nidogen-1;; unnamed protein product

          View more View less

          Gene Aliases: A630025O17; entactin; entactin-1; NID; nidogen-1

          View more View less

          UniProt ID: (Human) P14543, (Mouse) P10493

          View more View less

          Entrez Gene ID: (Human) 4811, (Mouse) 18073, (Rat) 25494

          View more View less

          Function(s)
          extracellular matrix structural constituent calcium ion binding collagen binding laminin binding laminin-1 binding proteoglycan binding extracellular matrix binding protein binding
          Process(es)
          cell-matrix adhesion positive regulation of cell adhesion positive regulation of muscle cell differentiation basement membrane organization regulation of basement membrane organization positive regulation of integrin-mediated signaling pathway positive regulation of cell-substrate adhesion extracellular matrix organization glomerular basement membrane development
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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