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Invitrogen
RGMA Recombinant Rabbit Monoclonal Antibody (24GB1320)
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FIGURE: 1 / 3
RGMA Antibody (MA5-57221) in ICC/IF
Immunocytochemical staining of RGMA in HepG2 cells using RGMA Recombinant Monoclonal Antibody (Product # MA5-57221) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; RGMA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-57221
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB1320
Immunogen
A synthesized peptide derived from human RGMA (aa 401-435)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.67 mg/mL
Amount
67 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: repulsive guidance molecule family member A; RGM domain family, member A; unnamed protein product
Gene Aliases: C230063O06; RGM
UniProt ID: (Human) Q96B86, (Mouse) Q6PCX7
Entrez Gene ID: (Human) 56963, (Mouse) 244058, (Rat) 308739
neural tube closure
positive regulation of protein phosphorylation
positive regulation of neuron projection development
negative regulation of neuron projection development
BMP signaling pathway
regulation of BMP signaling pathway
neuron projection development
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
negative regulation of collateral sprouting
negative regulation of axon regeneration
positive regulation of membrane protein ectodomain proteolysis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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